SKY is a 24-color, whole-chromosome painting assay that allows the simultaneous
visualization of each chromosome in different colors. Five spectrally distinct dyes
are used in combination to create a cocktail of probes unique to each chromosome.
The probe mixture is hybridized to metaphase chromosomes on a slide and then visualized
with a spectral cube, which allows the measurement of the entire emission spectrum
with a single exposure. The image is processed by computer software that can distinguish
differences in color not discernible to the naked eye by assigning a numerical value
to the RGB and pseudo color images.
SKY can detect chromosomal material of unknown origin, complex rearrangements, translocations,
large deletions, duplications, aneuploidy, and more. Some disadvantages of SKY include
ineffective detection of microdeletions and inversions. It can only be performed
on dividing cells.
Please inquire if your sample varies from the listed recommendations. Please inquire
for likely turnaround time.