Source:   A DNA sequence encoding the 165 amino acid residue variant of human VEGF (Leung, D.W. et al., 1989, Science 246:1306) was expressed in Sf 21 cells using a baculovirus expression system and the protein purified by sequential chromatography.

Purity & Assay Method:   >97%, as determined by SDS-PAGE, visualized by silver stain

Formulation:   Lyophilized from 62.1 µL of a 0.2 µm filtered solution in 30% acetonitrile, 0.1% TFA containing 50 µg of bovine serum albumin per 1 µg of cytokine

Mass Per Vial:   The vial size is 10 µg of recombinant human VEGF

Activity & Assay Method:   The biological activity of recombinant human VEGF was measured by its ability to stimulate ³H-thymidine incomporation in human umbilical vein endothelial cells (Conn, G. et al., Proc. Natl. Acad. Sci. USA, 87:1323-1327). The ED₅₀ for this effect is typically 1-6 ng/mL.

Reconstitution:   Reconstitute with sterile PBS containing at least 0.1% HSA or BSA. The rhVEGF basic concentration in the stock solution should be no less than 1 µg/mL.

Storage Conditions:   Lyophilized samples are stable for greater than six months at -20ºC to -70ºC. Upon reconstitution, this cytokine can be stored under sterile conditions at 2º to 4ºC for one month or at -20ºC to -70ºC for three months without detectable loss of activity. AVOID REPEATED FREEZE-THAW CYCLES.

Sterilization:   VEGF was filtered through a 0.2 micron membrane and packaged under aseptic conditions.

Endotoxin Level & Assay Method:   The endotoxin level was determined by the LAL method and found to be less than 0.1ng per 1µg of the cytokine.

CAUTION: FOR RESEARCH USE ONLY. NOT FOR DRUG OR DIAGNOSTIC USE. NOT FOR USE IN HUMANS.

NOTE: Recipients of bulk cytokines and monoclonal antibodies will be required to sign a Material Transfer Agreement. All International requesters are required to pay a packaging and handling fee. All recipients are required to pay for the shipper’s courier fee, therefore, a Fed Ex account number or UPS label is required.