NCI-Frederick Cryopreservation and Assisted Reproduction Laboratory

Cryopreservation and Assisted Reproduction Laboratory - Request for Services

Please note that a separate form needs to be completed for each strain.

Contact Information

Type of Service(s) Requested


Cryopreservation:	Embryo Sperm Ovary

Reproduction:	Colony Expansion Special Rederivation

Strain Reconstitution from:	Embryos Sperm Ovary

Current Location of Colony

Current location of colony:

Mice currently available:

Yes No

Contact person in animal facility:

Laboratory Animal Model to be Sent or Reconstituted

Species:

Strain Nomenclature:

Short Name:

Coat Color:

Age:

Transgenic Knockout Mutant

Strain Background:

Hybrid Inbred Mixed

Phenotypic Expression:

Is your strain genetically engineered?

Yes No

Does this construct contain a viral sequence or other potentially transposable agent?

Yes No

If yes, please describe:

Genotype:

Heterozygous Homozygous

Indicate best method to verify genotypic expression:

Sample preference for genotype verification (required):

Method of breeding and reproductive performance:

Special Requirements/ Notes:

PCR Information


Beginning Fiscal Year 2005, the cryopreservation and Assisted Reproduction laboratory will be offering genotyping by PCR that follows the quality control procedure for sperm, embryo, and ovary cryopreservation. This is to expedite the genotyping process and to reduce turn around time for all cryopreservation requests. For this purpose, please provide the primers along with the following information:
Sequence of the Primers:
Concentration of the Primers:
Melting Temperature (T_m), °C:
Expected size of the amplified fragment:
A representative picture of the PCR product:



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