Receiving and Quarantine - Timelines – Quarantine Rederivation Program

Donor Mice Required to Commence Rederivation:

	4 Males that are 8-15 weeks of age
	8 Females that are 3-4 weeks of age
	Please note that additional donors may be required if no litters are successfully rederived or if the donor animals provided are too old for breeding

The Time Required to Build a Colony and Distribute Mice Depends on:

	How rapidly the strain is transferred to NCI-Frederick
	The number of mice transferred by the donating investigator
	The age of the mice transferred
	Strain Fecundity
	Whether IVF (in vitro fertilization) is an option for colony expansion

The Following Chart Was Constructed Using these Assumptions:

	Target Colony Size = 15 breeder pairs
	Generation Time = 12 weeks
	Average Litter Size = 6
	Male and female produced equally
	Both males and females may be used and distributed as breeders
	Adequate space in Receiving and Quarantine (R&Q) for large-scale IVF

Please contact the Receiving and Quarantine Facility at 301-846-1133 if you have any questions regarding this information.