Transgenic and Knock-Out Services - Expertise

The Transgenic Mouse Model

The Transgenic Mouse Model (TMM) Laboratory provides assistance to NCI investigators with a need to study in vivo gene function, by producing transgenic mice by pronuclear microinjection of fertilized mouse eggs. Although this service is customized to the needs of each client, the core of this effort includes purification of the DNA fragment to be microinjected and analysis of the resulting mice by Southern blot, PCR, or slot/dot blot. Consultations for the design of recombinant DNA constructs for the use in transgenic mouse studies are available. Characterization of the pattern of transgene expression at the RNA or protein level is also available. Transgenic mice are routinely produced in FVB/N and C57BL/6 backgrounds. Click here for a list of investigators who have used TMM services in CY2002-2003.