Laboratory Animal Sciences Program
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Definitions

Antibody development:Optimize the techniques for the visualization of a new antibody.

Bioassay/phenotyping necropsy:a full and complete necropsy with examination and collection of all organs.

Blood Collection:Sampling blood from various anatomic sites for hematology and/or blood chemistry analysis. Please call for details.

Complex serial sectioning: a method of serial sectioning which allows sequential sections at one level of the tissue to be represented on multiple slides (this is useful for multiple stain techniques).

Double Stain Technique:a technique in which 2 or more antibody stains are applied to a tissue section simultaneously.

Early to late stage embryo removal:at 6.5 days post-conception (early) up to 17 to 18 days post-conception (late).

Fixation:The stabilization of protein. Fixatives, such as, Bouins, 10% Neutral Buffered Formalin, 4% Paraformaldehye, and 70% Ethanol are chemicals that alter tissues by stabilizing protein. The goal of fixation is to make tissues resistant to any further changes.

Gross Photography:Photography of necropsy specimens. Capturing images of whole animal or organ; usually performed at time of necropsy.

Hematology:Preparation of blood smears and CBC (complete blood counts).

Immunohistochemistry Fluorescence: the use of fluorescent markers to visualize primary antibody localization on tissue sections.

Inguinal:Relating to the left or right ventral region where the abdomen connects with the rear legs (also know as the “groin”) of the abdomen/groin.

Mandibular lymph node: bilateral lymph nodes immediately adjacent to the salivary glands in rodents.

Nasal sections:nasal turbinates, sinuses, teeth, eyes, Harderian glands, and skull.

Organ Weights:the mass of an organ from a necropsied specimen.

Resins:Any of numerous physically similar polymerized synthetics or chemically modified natural substances including thermoplastic materials such as polyvinyl, polystyrene, and polyethylene and thermosetting materials such as polyesters, epoxies, and silicones that are used with fillers, stabilizers, pigments, and other components to form plastics.

Serial Sectioning:Microtomy technique requiring every section that is cut to be mounted onto a slide (usually used for identifying small targets or critical analysis of specific tissue).

Sterile:Free from all live bacteria or other microorganisms and their spores.

Step Sectioning:a number of pre-specified sections that are discarded
between sections that are kept (useful to obtain a reasonable representation of specific tissue or broad targets).

Tissue Collection: Removal of organs at time of necropsy.

Whole Body Perfusions:Introduction of a fixative into the body through the circulatory system (an alternate method of fixing the tissue).

 

 

 

 

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