Laboratory Animal Sciences Program
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Fixatives and Solutions

Routine fixatives:

  • 10% Neutral Buffered Formalin (NBF)
    • 37 - 40% Formaldehyde………………………………………1000mL
    • distilled water ……………………………………………9000mL
    • sodium phosphate monobasic …………………40grams
    • sodium phosphate dibasic (anhydrous)……65grams

  • Bouin's
    • Picric acid (saturated aqueous solution)………750mL
    • 37 - 40% Formaldehyde …………………………………………250mL
    • Glacial acetic acid ……………………………………………50mL

  • 4% Paraformaldehyde
    • Paraformaldehyde ……………………40grams
    • Deionized water…………………………500mL
    • 10N Sodium Hydroxide………………20uL
    • 10X PBS ………………………………………100mL
    • Q.S. with deionized water …………400mL

  • 100% Ethanol
    • Ethanol ………………………………………11200mL
    • Distilled water………………………………4800mL

  • Carnoy 's
    • 100% Ethanol …………………………………600mL
    • Chloroform……………………………………30mL
    • Glacial acetic acid ……………………………………100mL

  • Davidson
    • Glacial acetic acid ………………………………………100mL
    • 95% Ethyl alcohol…………………………………………300mL
    • 10% Neutral Buffered Formalin (NBF)…………200mL
    • Distilled water………………………………………………300mL

  • Zinc Formalin
    • Distilled water………………………………………2000mL
    • Formalin ………………………………………………50mL
    • Zinc chloride…………………………………………15grams

  • Telley's
    • 100% Ethanol ……………………………………700mL
    • Formaldehyde…………………………………50mL
    • Glacial acetic acid……………………………50mL
    • Distilled water…………………………………200mL

Other Solutions

  • Formic Acid Sodium Citrate Decal
    • Distilled water……………………………………1500mL
    • Sodium citrate, dehydrate…………………200mL
    • Formic acid …………………………………………500mL

     

  • EDTA Decal

    • Distilled water…………………………………3000mL
    • EDTA…………………………………………………200grams
    • Sucrose ……………………………………………280grams

 

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