Laboratory Animal Sciences Program
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Image Analysis

PHL offers complete qualitative and quantitative analysis of samples ranging from whole mice to specific anatomical microenvironments. Comprehensive reports with raw and processed data are presented to the researcher along with all the images used to conduct the analysis.


Image Analysis

Provides a comprehensive qualitative and quantitative analysis of whole animals, organs specific, and/or anatomical features. Whether looking at a single population of cells, comparing multiple populations of cells, examining organs from control animals and transgenic animals, or demonstrating percent of metastasis in a single organ, there is nearly a limitless amount of information that can be obtained. A library of routine analysis techniques is maintained in addition to working closely with researchers to develop novel techniques yielding the most robust and useful information for their study(s).

•  Analysis software

•  Image-Pro ® Plus v5.1

 click + or - Information obtained

  • Number
  • Area (relative and absolute)
  • Diameter, radius
  • Dimensions (Length, Width, Height)
  • Fragmentation
  • Percent composition
  • Proximity
  • Intensity (bright-field and fluorescence)

 click + or - “Macro” Creation and Implementation

Macros are custom designed sub-routines that execute procedures with in the software that would take considerable time if done for each image by the user. Several standard macros have been constructed and saved in PHL's macro library for routinely run analyses. For specialized cases, personalized macros can be constructed to yield data pertinent to the study.

 click + or - Current Routine Macros

  • CD31 (micro-vessel marker)
  • PCNA (proliferating cell nuclear marker)
  • Apoptosis (apoptotic nuclear marker)
  • Generalized membrane marker

 click + or - Pseudo-Random Image Analysis (sample imaging)

Images for analysis are pseudo-randomly shot about the tissue typically with a 20x objective. The images are then archived, edited as needed, and run through the count macro.

 click + or - Whole Tissue Anatomical Analysis

Entire tissues are analyzed at once as opposed to sampling images throughout the tissue. This provides a more “true to life” representation of the sample. All potential “signals” are accounted for including any hotspots and low expression areas that may be missed through sample imaging.

Image Capture

Provide publication quality images of whole animals, organs, tissues, or cell preparations. With a comprehensive understanding of optics, photography, microscopy, histology, and pathology, the knowledgeable PHL staff can assist in providing high quality images. Images can be provided in numerous formats including, jpg, tiff, raw, psd, and others.

 click + or - Bright Field Microscopy

High quality, high resolution images suitable for analysis and subsequent publication.

 click + or - Epifluorescent Microscopy

Clean, sharp images free of background auto-fluorescence. Ideal solution for dual-labeling immunofluorescence experiments.

 click + or - Whole tissue slide scanning

Entire tissues on slides scanned at up to 40X magnification without stitching. High resolution, even edge-to-edge illumination.

•  Whole animal/organ photography


Image Enhancement

Essential enhancements for digital images including straightening, white balance adjustments, cropping, and sharpening. Additional services include creating collages, annotation capabilities, and novel post-capture image adjustments to complement the unprocessed image.

 click + or - Software

  • Adobe ® Photoshop CS2
  • Image-Pro ® Plus v5.1

Professional Capabilities:

The PHL staff are able to combine expertise in image analysis; (photography, microscopy, software involved) with the steps that lead up to the finished product (anatomy, histology, IHC, IF). In addition, veterinary pathologists provide invaluable assistance to ensure maximum scientific integrity.

 

 

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