Laboratory Animal Sciences Program
LASP logo   NCI-Frederick
PHL PHL banner


Microtomy is Cutting Edge Work! It is the area of the laboratory, where blocks (usually in paraffin) are sectioned to produce slides that can be evaluated microscopically. A level of expertise rarely found anywhere else. These expertise include, but are not limited to:

  • Complex serial and step sectioning
    • On mice and other organisms
  • Cutting of plastic resins
  • Cutting of extremely thick or thin sections (2 µ to 1000 µ)
  • Routine Hematoxylin and Eosin slides (H & E’s): great quality slides are done in a timely manner

Our expert histologists are able to zero-in on small details and minute structures.

  • Also specializing in rodent embryos, can target specific locations following the M.H. Kaufmann’s "The Atlas of Mouse Developement"

Cryotomy – Frozen Sectioning

Frozen or fixed sections are used as an alternative to formalin fixed paraffin embedded (FFPE) tissues. Frozen sections are used when the normal fixation/processing regime may denature the target. Examples where this may be important are:

  • Demonstration of lipids
  • Enzyme histochemistry
  • Beta Galactosidase
  • Immunohistochemistry
  • Special stains

  National Cancer Institute (NCI)      National Institutes of Health (NIH)      Department of Health and Human Services (HHS)  
     Privacy Policy  |  Developed by C&SS |  Internet Toolkit