Trimming and Embedding
Trimming consists of cutting 3-4 mm thick representative sections of requested tissues following specialized trim protocols with detailed orientation instructions of each tissue. The trimmed tissues are then placed in uniquely identified cassettes for processing into paraffin blocks. An average of 3 different tissues or sections of one tissue are embedded per block. Our routine trim protocol is the mouse bioassay/phenotyping protocol.
Paraffin embedding requires a process of dehydration and infiltration. This process is normally carried out on an automated Vacuum Infiltration Processor. This laboratory also uses microwave processing and a range of hand processing protocols for early stage embryos and delicate tissues.
Processed tissues are embedded in paraffin using standard orientation procedures.