Available Strain Details

Strain Information
Common Strain Name: Msh2 null Pricing and Ordering Information
Strain Nomenclature: B6.129-Msh2tm1Rak/Nci
Animal State: Cryoarchived Strains
Release Category (Required for MTA form): B1


Strain Description: This strain carries a null mutation in the DNA mismatch repair gene Msh2. Cells from mice homozygous for this mutation are incapable of repairing single-nucleotide and insertion/deletion mismatches. Homozygous mice become moribund between 2.5 and 11.5 months, the result of lymphomas (about 70%) and/or intestinal tumors (about 70%). Heterozygous mice have a higher incidence of tumors than wild type mice, but live in excess of 16 months. This strain has been used to study the effect of this mismatch repair mutation on mice carrying Apc mutations.
Mutation Information
Mutation Type: Targeted
Gene Name: MutS homolog 2
Gene Symbol: Msh2
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.129
Approx. Generation: 12
Organ Site: GI
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C57BL/6 (male or female).
Genotyping Information: Protocol 1: Allele: Msh2<tm1Rak>
Donating Investigator: Dr. Raju Kucherlapati

Key Reference: Smits R, Hofland N, Edelmann W, Geugien M, Jagmohan-Changur S, Albuquerque C, Breukel C, Kucherlapati R, Kielman MF, Fodde R. Somatic Apc mutations are selected upon their capacity to inactivate the beta-catenin downregulating activity. 2000. Genes, Chromosomes & Cancer 23:229-23 PMID: 10992298 [Pubmed Abstract]