Available Strain Details

Strain Information
Common Strain Name: Gadd45a null Pricing and Ordering Information
Strain Nomenclature: B6;129-Gadd45atm1Ajf/Nci
Animal State: Cryoarchived Strains
Release Category (Required for MTA form): B1


Strain Description: By homologous recombination a 3.65 kb segment of the Gadd45a gene, including upstream sequences and exons 1-3, were replaced with a PGK-neo cassette. Homozygous Gadd45a-deficient mice were born at Mendelian frequency and grew to adulthood normally. One year after 6-8 week-old mice had been subjected to ionizing radiation, the frquency of thymic lymphomas was 3x higher than in wild-type controls. Cell from Gadd45a-deficient mice show chromosomal instability. Approximately 1/2 of Gadd45a-deficient (homozygous) females in a mixed 129:B6 background did not breed successfully. Successful delivery in most in a pure B6 background.
Mutation Information
Mutation Type: Targeted
Gene Name: Gadd45a
Gene Symbol: Gadd45a
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6;129
Approx. Generation: 19
Organ Site: Hematopoietic
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x heterozygous males. When maintained as a live colony, breeder pairs were distributed as a heterozygote (male or female) x wildtype C57BL/6 (male or female). Average litter size is six. 50% of homozygous females in mixed 129;B6 background died of delivery failure. This trait appears to not be present in the pure C57BL/6 background.
Genotyping Information: Protocol 1: Allele: Gadd45a<tm1Ajf>
Donating Investigator: Dr. Albert Fornace, Jr.

Key Reference: Hollander MC; Sheikh MS; Bulavin DV; Lundgren K; Augeri-Henmueller L; Shehee R; Molinaro TA; Kim KE; Tolosa E; Ashwell JD; Rosenberg MP; Zhan Q; Fernandez-Salguero PM; Morgan WF; Deng CX; Fornace AJ Jr. Genomic instability in Gadd45a-deficient mice. Nat Genet 23, 176-184, 1999. PMID 10508513 [Pubmed Abstract]

Related Reference: Hollander MC, Philburn RT, Patterson AD, Wyatt MA, Fornace AJ Jr. Genomic instability in Gadd45a-/- cells is coupled with S-phase checkpoint defects. Cell Cycle 4, 704-709, 2005. PMID 15846075. [Pubmed Abstract]

Hollander MC, Patterson AD, Salvador JM, Anver MR, Hunger SP, Fornace AJ. Gadd45a acts as a modifier locus for lymphoblastic lymphoma. Leukemia 19, 847-850, 2005. PMID 15744335. [Pubmed Abstract]

Hollander MC, Kovalsky O, Salvador JM, Kim KE, Patterson AD, Haines DC, Fornace AJ Jr. Dimethylbenzanthracene carcinogenesis in Gadd45a-null mice is associated with decreased DNA repair and increased mutation frequency. Cancer Res 61, 2487-2491, 2001. PMID 11289119. Hollander MC, Kovalsky O, Salvador JM, Kim KE, Patterson AD, Haines DC, Fornace AJ Jr. Dimethylbenzanthracene carcinogenesis in Gadd45a-null mice is associated with decreased DNA repair and increased mutation frequency. Cancer Res 61, 2487-2491, 2001. PMID 11289119. [Pubmed Abstract]

Sponsor: NIH Intramural Program