Available Strain Details

Strain Information
Common Strain Name: Ink4a/Arf null (B6) Pricing and Ordering Information
Strain Nomenclature: B6.129-Cdkn2atm1Rdp/Nci
Animal State: Cryoarchived Strains
Release Category (Required for MTA form): C3


Strain Description: This strain carries a targeted deletion of exons 2/3 of the INK4a/ARF locus which eliminates both p16 (Ink4a) and p19 (Arf). Mice homozygous for this deletion are viable and fertile, but exhibit a cancer-prone condition. On a mixed genetic background, spontaneous development of various tumors (primarily B-cell lymphomas and sarcomas) is observed by ~6 months of age. Tumors arisen in mice heterozygous for this deletion require a longer latency and uniformly demonstrate loss-of-heterozygosity of the second allele. On a C57BL/6J background, tumor latency is prolonged. In addition to the tumor phenotype, these mice, by two months of age, histologically exhibit mild proliferative expansion of the white and red pulp of the spleen. Mice older than 6 months develop more severe extramedullary hematopoesis in the spleen and other lymphoid organs such as the liver.
Mutation Information
Mutation Type: Targeted
Gene Name: Cyclin-dependent kinase inhibitor 2A
Gene Symbol: Cdkn2a
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6.129
Approx. Generation: Homo
Organ Site: All
Additional Information
Cryopreservation Mating Scheme: Multiple freezes were done for this strain; The cryopreservation mating scheme is homozygous males x C57BL/6 females OR homozygous x homozygous. The vials/straws are identified accordingly. When maintained as a live colony, breeder pairs were distributed as homozygotes.
Genotyping Information: Protocol 1: Allele: Cdkn2a<tm1Rdp>
Donating Investigator: Dr. Ron DePinho

Key Reference: Manuel Serrano, Han-Woong Lee, Lynda Chin, Carlos Cordon-Cardo, David Beach, and Ronald A. DePinho. Role of the INK4a Locus in Tumor Suppression and Cell Mortality. 1996. Cell 85: 27. PMID: 8620534 [Pubmed Abstract]