Available Strain Details

Strain Information
Common Strain Name: LANA Pricing and Ordering Information
Strain Nomenclature: B6.129-Tg(HHV8ORF73,Tk-GFP)1Dtmr/Nci
Animal State: Cryoarchived Strains
Release Category (Required for MTA form): B1


Strain Description: Kaposi sarcoma-associated herpesvirus (KSHV) is a human lymphotropic herpesvirus. It is implicated in B cell neoplasias such as primary effusion lymphoma and multicentric Castleman disease in AIDS patients. The KSHV latency-associated nuclear antigen (LANA) is consistently expressed in all KSHV-associated tumor cells and was shown to bind the tumor suppressor proteins p53 and pRb. To test LANA's contribution to lymphomagenesis in vivo we generated transgenic mice expressing LANA under the control of its own promoter, which is B cell specific. All of the transgenic mice developed splenic follicular hyperplasia due to an expansion of IgM+ IgD+ B cells and showed increased germinal center formation. We also observed lymphomas (latency 300 days), implying that LANA can activate B cells and provide the first step toward lymphomagenesis.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: LANA
Transgene Symbol: LANA
Promotor Name: LANA, KSHV (viral)
Promotor Symbol: LANA, KSHV
Current Genetic Background: B6.129
Approx. Generation:
Organ Site: Hematopoietic
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x hemizygous males.
Genotyping Information: Protocol 1: Allele: Tg(HHV8ORF73, Tk-GFP)1Dtmr
Donating Investigator: Dr. Dirk Dittmer

Key Reference: Fakhari FD, Jeong JH, Kanan Y, Dittmer DP. The latency-associated nuclear antigen of Kaposis sarcoma-associated herpesvirus induces B cell hyperplasia and lymphoma. J Clin Invest 3, 735-742, 2006. PMID: 16498502 [Pubmed Abstract]

Sponsor: NIH R01 CA109232-03