Available Strain Details

Strain Information
Common Strain Name: Nkx3.1-CreERT2 Pricing and Ordering Information
Strain Nomenclature: B6;129S-Nkx3-1tm4(cre/ERT2)Mms/Nci
Animal State: Cryoarchived Strains
Release Category (Required for MTA form): C4A,C4B


Strain Description: This allele is a knock-in of an inducible Cre recombinase into the Nkx3.1 locus (and is a null allele), and can be used for inducible gene targeting in the prostate epithelium.Homozygous males should be bred at a young age (within the first 4-5 months). Occasionally, if not bred early on, they do not mate; some homozygous males never breed. If there is a breeding cage that has not had pups for 2 months, the male should be removed and a new one put in. Once they start breeding they do fine. Average litter size is 6-8.
Mutation Information
Mutation Type: Targeted
Gene Name: NK-3 transcription factor, locus 1 (Drosophilia)
Gene Symbol: Nkx3-1
Transgene Name:
Transgene Symbol:
Promotor Name:
Promotor Symbol:
Current Genetic Background: B6;129S
Approx. Generation: 11
Organ Site: Prostate
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is homozygous x homozygous. When maintained as a live colony, breeder pairs were distributed as homozygotes. Homozygous males should be bred at a young age (within the first 4-5 months). Occasionally, if not bred early on, they do not mate; some homozygous males never breed. If there is a breeding cage that has not had pups for 2 months, the male should be removed and a new one put in. Once they start breeding they do fine. Average litter size is 6-8.
Genotyping Information: Protocol 1: Allele: Nkx3-1<tm4(cre/ERT2)Mms>
Donating Investigator: Dr. Michael Shen

Key Reference: Wang, X., Kruithof-de Julio, M., Economides, K. D., Walker, D., Yu, H., Halili, M. V., Hu, Y.-P., Price, S. M., Abate-Shen, C., and Shen, M. M. (2009). A luminal epithelial stem cell that is a cell of origin for prostate cancer. Nature 461: 495-500. PMID: 19741607 [Pubmed Abstract]

Sponsor: U01 CA084294 (NCI Mouse Models of Human Cancers Consortium), R01 DK076602