Available Strain Details

Strain Information
Common Strain Name: RIP1-Tag2 (B6) Pricing and Ordering Information
Strain Nomenclature: B6.D2-Tg(RIP1-Tag2)2Dh/Nci
Animal State: Cryoarchived Strains
Release Category (Required for MTA form): A,D

Strain Description: In this strain the rat insulin promoter (RIP) directs expression of the SV40 Large T antigen transgene (TAg) to beta cells of the pancreatic islets. The SV40 TAg oncogene is expressed beginning at ~E8.f. The mice are systemically tolerant to large T antigen. Hyperplastic islets begin to appear by 3-4 weeks of age. Angiogenic islets (8 to 12% of the total) arise from hyperplastic/dysplastic islets by switching on angiogenesis. Solid tumors (~3% of the islets) emerge at about 10 weeks as small, encapsulated adenomas that progress into large adenomas by 12 to 13 weeks. Development to vascularized invasive carcinomas occurs less frequently. This model has many virtues as a prototype for testing experimental therapeutics, although it will not necessarily be suitable for all drugs, such as oncogene-specific inhibitors. The advantages of the model are its 14-week time course, the 100% penetrance of invasive cancer, and the synchronous appearance of dysplasias, angiogenic switching, adenomas, and carcinomas. The multifocality of the target tissue (the 400 islets) and the lesions that develop allow quantification of tumor development. When maintained as a live colony, breeder pairs were supplied as a hemizygote (male or female) x C57BL/6N mate. This strain was maintained as a continuous backcross to C57BL/6 for 20+ generations. This strain arose from the microinjection of B6D2F2 embryos. The integration site and the strain-of-origin chromosome are unknown. If the integration site was within a C57BL/6 chromosome then the genetic background could be pure C57BL/6. Wild type littermates may be used in the breeding scheme.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: SV40 Large T antigen
Transgene Symbol: TAg
Promotor Name: Rat Insulin Promoter
Promotor Symbol: RIP
Current Genetic Background: B6.D2
Approx. Generation: 53
Organ Site: GI
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype C57BL/6 (male or female); however, wlldtype littermates may be used in the breeding scheme.
Genotyping Information: Protocol 1: Allele: Tg(RIP1-Tag2)2Dh
Donating Investigator: Dr. Doug Hanahan

Key Reference: Hanahan D. 1985. Heritable formation of pancreatic beta-cell tumors in transgenic mice expressing recombinant insulin/simian virus 40 oncogenes. Nature 315:115-22 PMID: 2986015 Adams TE, Alpert S, Hanahan D. 1987. Nono-tolerance and autoantibodies to a grangenic self antigen expressed in pancreatic beta cells. Nature 325:223-8. [Pubmed Abstract]