Available Strain Details

Strain Information
Common Strain Name: Fabp1-Cre Pricing and Ordering Information
Strain Nomenclature: FVB/N-Tg(Fabp1-Cre)1Jig/Nci
Animal State: Cryoarchived Strains
Release Category (Required for MTA form): B1,D


Strain Description: Cre is expressed in the intestinal epithelium with greatest expression occurring in the distal small intestine and colon. The multipotent gut stem cell and descendant cell lineages all support Cre-mediated recombination as judged by crosses of Tg/+ mice to mice heterozygous for a floxed R26R (ROSA26) reporter locus. Note that recombination is mosaic, with some but not all crypt-villus units in the small bowel and some but not all crypts in the colon showing R26R recombination (see Wong et al, Proc. Natl. Acad. Sci. USA 97:12601 [2000]). The number of crypts with recombination increases along the duodenal-colonic axis. Extraintestinal sites of expression are limited to the urothelium where Cre expression is uniform throughout. Cre expression in the intestinal epithelium is initiated as early as E13.5 and in the urothelium as early as E16.5. Expression is sustained through adulthood.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: Cre recombinase
Transgene Symbol: Cre
Promotor Name: Fatty acid binding protein (rat)
Promotor Symbol: Fabp1
Current Genetic Background: FVB/N
Approx. Generation: 40
Organ Site: GI
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype FVB (male or female). Wildtype littermates may be used in mating.
Genotyping Information: Protocol 1: Allele: Tg(Fabp1-Cre)1Jig
Donating Investigator: Dr. Jeffrey Gordon

Key Reference: Wong, MH, Gordon, JI 2000. Genetic mosaic analysis based on Cre recombinase and navigated laser capture microdissection. Proc. Natl. Acad. Sci. USA 97:12601-06 PMID: 11050178 [Pubmed Abstract]

Related Reference: Saam JR, Gordon JI. 1999. Inducible gene knockouts in the small intestinal and colonic epithelium. J. Biol Chem. 274:38071-82. PMID: 10608876 [Pubmed Abstract]