Available Strain Details

Strain Information
Common Strain Name: PB-Cre4 Pricing and Ordering Information
Strain Nomenclature: B6.Cg-Tg(Pbsn-cre)4Prb/Nci
Animal State: Cryoarchived Strains
Release Category (Required for MTA form): B1,D


Strain Description: Cre expression is post-natal and is specific to prostrate epithelium. Expression is highest in the lateral lobe, followed by the ventral lobe and then the dorsal and anterior lobes. There is limited focal cre activity in the seminiferous tubules and the ovary. There is low-level expression of cre in oocytes. Male PB-Cre4 mice must be used to transmit the cre transgene to ensure tissue-specific recombination. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x C57BL/6N mate. Hemizygote 01XF5 females can be used as breeders to maintain a colony. Issues arise when 01XF5 mice are crossed with floxed mutant animals to generate tissue-specific knockouts. There is low-level expression of Cre in oocytes of 01XF5 mice, so when 01XF5 females are crossed to floxed mutants, recombination may occur in all tissues. To achieve recombination specifically in the prostate epithelium it is essential that one uses a MALE 01XF5 mouse and crosses it to a female mouse of the floxed strain.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: Cre recombinase
Transgene Symbol: Cre
Promotor Name: Probasin (rat)
Promotor Symbol: Pbsn
Current Genetic Background: B6.Cg
Approx. Generation: 40
Organ Site: Prostate
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is C57BL/6 females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype C57BL/6 (male or female).

Hemizygote 01XF5 females can be used as breeders to maintain a colony. Issues arise when 01XF5 mice are crossed with floxed mutant animals to generate tissue-specific knockouts. There is low-level expression of Cre in oocytes of 01XF5 mice, so when 01XF5 females are crossed to floxed mutants, recombination may occur in all tissues. To achieve recombination specifically in the prostate epithelium it is essential that one uses a MALE 01XF5 mouse and crosses it to a female mouse of the floxed strain.
Genotyping Information: Protocol 1: Allele: Tg(Pbsn-Cre)4Prb
Donating Investigator: Dr. Pradip Roy-Burman

Key Reference: Wu X, Wu J, Huang J, Powell WC, Zhang J, Matusik RJ, Sangiorgi FO, Maxson RE, Sucov HM, Roy-Burman P. 2001 Generation of a prostate epithelial cell-specific Cre transgenic mouse model for tissue-specific gene ablation. Mech Dev 101:61-69. PMID: 11231059 [Pubmed Abstract]