Available Strain Details

Strain Information
Common Strain Name: HB-Plap(G11) Pricing and Ordering Information
Strain Nomenclature: FVB-Tg(ACTB-ALPP)02Jrst/Nci
Animal State: Cryoarchived Strains
Release Category (Required for MTA form): C1


Strain Description: Mutant human PLAP (ALPP) gene with 7Gs inserted downstream of a 4 G run resulting in a frameshift that yields a non-functional PLAP gene product. The transgenic mouse reports frameshift mutations by reverting the PLAP transgene to wildtype. Histological assay of heat stable alkaline phosphatase activity detects frameshift mutations in all tissues of the animal. Useful for detecting mutation distribution resulting from genetic defects and environmental exposure to mutagens.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: human placental alkaline phosphatase
Transgene Symbol: PLAP
Promotor Name: human beta actin
Promotor Symbol: ACTB
Current Genetic Background: FVB
Approx. Generation: 5
Organ Site:
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype FVB (male or female).
Genotyping Information: Protocol 1: Allele: Tg(ACTB-ALPP)02Jrst
Protocol 2: Allele: Tg(ACTB-ALPP)02Jrst
Donating Investigator: Dr. James Stringer

Key Reference: Hersh MN, Stambrook PJ, Stringer JR 2002 Visualization of mosaicism in tissues of normal and mismatch-repair- deficient mice carrying a microsatellite-containing transgene. Mutat Res 505:51-62. PMID: 12175905 [Pubmed Abstract]

Related Reference: DePrimo SE, Cao J, Hersh MN, and Stringer JR. 1998 Use of human placental alkaline phosphatase transgenes to detect somatic mutation in mice in situ. Methods 16: 49-61. PMID: 9774516 [Pubmed Abstract]

Cao J, DePrimo SE, Hersh MN, Stringer JR. In situ detection of frameshift mutations in mouse cells. Mutat Res. 1998 Nov 3;421(2):163-78. PMID: 9852990 [Pubmed Abstract]