Available Strain Details

Strain Information
Common Strain Name: 76 T2/Onc (Sleeping Beauty) Pricing and Ordering Information
Strain Nomenclature: FVB-TgTn(sb-T2/Onc)76Dla/Nci
Animal State: Cryoarchived Strains
Release Category (Required for MTA form): C1


Strain Description: 76 T2/Onc mice carry Sleeping Beauty transposon concatemers on chromosome 1. Together with a strain expressing the Sleeping Beauty transposase, CAGGS-SB10 (NCI Mouse Repository strain 01XN7), these mice can be used for unbiased, forward genetic screens for cancer genes in vivo.
Mutation Information
Mutation Type: Transgenic
Gene Name:
Gene Symbol:
Transgene Name: sleeping beauty transposon (mouse)
Transgene Symbol: sb
Promotor Name: MSCV LTR (viral)
Promotor Symbol: MSCV LTR (viral)
Current Genetic Background: FVB
Approx. Generation:
Organ Site: Various
Additional Information
Cryopreservation Mating Scheme: The cryopreservation mating scheme is FVB females x hemizygous males. When maintained as a live colony, breeder pairs were distributed as a hemizygote (male or female) x wildtype FVB mate (male or female). Average litter size is 6-8.
Genotyping Information: Protocol 1: Allele: TgTn(sb-T2/Onc)76Dla
Donating Investigator: David Largaespada

Key Reference: Collier LA, Carlson CM, Ravimohan S, Dupuy AJ, Largaespada DA. Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse. Nature 436, 272-276, 2005. PMID: 16015333. [Pubmed Abstract]

Sponsor: NIH-NIDA, R01 (DA014764), 2/15/03 - 11/30/05, NIH-NCI, U01 (CA84221, Years 6-10), 2/15/03 - 3/31/09