Mathematical Analysis of Biological Datasets
Our capabilities include, but are not
- Biomarker Discovery
- Genome-Wide Association Studies
- Survival Analysis
- Power Calculations for Study Design
- Statistical Analysis
Contact Information: Dr. Brian T. Luke (301)846-5553
Factors that Affect Donated Tissue Quality
The GTEx program plans to collect ?non-diseased? tissues from 900 donors for further analysis
by the end of fiscal year 2015. During the pilot phase, as per the study goal, a total of 190 cases
were collected. To refine the requirements for Scale up, many of the processes utilized in the GTEx
pilot such as quality of the collected biospecimens and accuracy of collected data were evaluated
to determine their ability to scale and to collect high quality specimens and data to meet the project goals.
This work was done in collaboration with Dr. Anita H. Undale, OBBR, Leidos Biomedical Research, Inc.
Quality (RIN) vs Collection Time (PMI)Adrenal Gland (r = -0.767)
Proteomic Changes Resulting from Irradiation
In collaboration with Dr. Deborah E. Citrin, Radiation Oncology Branch, CCR,
this project examined changes in the extracellular proteome using LC/MS/MS techniques.
Suction was used to extract blister fluid from individuals immediately before irradiation,
immediately after irradiation, and one year later. Differentially expressed genes were
identified using in-house software, and then processed using DAVID to elucidate common ontologies.
Results comparing immediately before and after irradiation
|GO:0030529~ribonucleoprotein complex||3.36E-09||Q12906, Q12905,
|GO:0003729~mRNA binding||1.91E-06||P27797, Q08257,
|GO:0003723~RNA binding||5.08E-06||Q12906, Q12905,
|RNA binding||3.92E-05||Q12906, P06748,