Lot Number: 09/2000
Source: The starting material was the filtrate from
mammalian cell culture. It was filtered to remove cell debris, followed by passage
through a rProtein A column to purify the murine Fc-endostatin. The dialyzed solution
was subsequently passed through a Q column for endotoxin removal. The protein solution
was sterile-filtered with a 0.20 um filter before vialing. The protein solution
was then dialyzed against 1xPBS to remove excess salt.
Purity & Assay Method: 99.1% by size exclusion chromatography.
Formulation: PBS, pH 7.4
Mass Per Vial: There are 2 vial sizes: 1 mg and 5
Storage Conditions: Store at -70oC
Activity: Inhibits cord formation with an IC50 of
7ug/ml and IC100 of 8ug/ml. It has no effect on proliferation, chemotaxis (HUVEC)
and on angiogenesis of chick aortic arch.
Sterility: Filtered through a 0.20 micron membrane
and packaged under aseptic conditions.
Endotoxin Level: The endotoxin level is 0.142 EU/ml.
CAUTION:FOR RESEARCH USE ONLY. NOT FOR DRUG OR DIAGNOSTIC USE. NOT FOR USE
NOTE: Recipients of bulk cytokines and monoclonal antibodies will be