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PHL Murine Immunohistochemistry Database

Notes on Techniques, Abbreviations, and Acronyms:

The following key to abbreviations used in the list assumes some basic knowledge of immunohistochemistry.

Mouse monoclonal antibodies on mouse tissues: Use of DAKO's ARK or Vector's MOM kits can reduce background staining when mouse monoclonals are used on mouse tissues. There may be other kits and other methods available that are not listed here.

Many of the techniques may be affected by the type or duration of the fixation used. Please contact us with questions regarding fixation or to request additional information.

Abbreviations

General
4C 4° celcius
60’ 60 minutes
AR Citra Antigen Retrieval Citra (citrate buffer) by BioGenex
dH2O distilled water
EDTA Ethylenediaminetetracetic acid
HR. Hour
MW microwave
no MW no microwave
no Tr. no Trypsin
OCT Fresh frozen cryostat sections
ON overnight
PC pressure cooker
RT room temperature
Tr. Trypsin
 
Chromagen/Substrate
AP Alkaline Phosphatase
DAB 3,3’-Diaminobenzidine Tablets by Sigma, Liquid by DAKO
DAPI DAPI by VECTOR
HRP Horseradish Peroxidase
Nova Red Nova Red Substrate Kit, SK-4800 by VECTOR
Texas Red Texas Red Avidin D by VECTOR
VECTOR Blue Alkaline Phosphatase Substrate Kit III, SK-5300, by VECTOR
VECTOR Red (AP Red) Alkaline Phosphatase Substrate Kit I, SK-5100, by VECTOR
 
Kits
ABC only Avidin – Biotinylated Enzyme Complex Only; used when biotinylated primary antibodies are applied to tissue, VECTOR
ApopTag Apoptosis Detection Kit, Millipore
ARK Animal Research Kit, DAKO
Fluorescein 2Ab Fluorescein a/Rabbit IgG (H+L), affinity purified, made in goat, VECTOR
Goat e. Goat Elite Kit, VECTOR
Guinea Pig Guinea Pig Kit, VECTOR
Human e. Human Elite Kit, VECTOR
LSAB+ LSAB+ (labelled streptavidin biotin) Kit, DAKO
M.O.M. Mouse primary antibody On Mouse Tissue Kit, VECTOR
Mouse e. Mouse Elite Kit, VECTOR
Mouse e./F(ab’)2/m Blocking serum and Avidin-Biotinylated complex from Mouse Elite Kit, VECTOR; secondary antibody is biotinylated a/mouse Immunoglobulins (Biotin Rabbit F(ab’)2), DAKO
NDS Normal Donkey Serum
NGS Normal Goat Serum
NHS Normal Horse Serum
NRS Normal Rabbit Serum
Rabbit e. Rabbit Elite Kit, VECTOR
Rat e. Rat Elite Kit, Vector
Rat e./2Ab mouse adsorbed Blocking serum and Avidin-Biotinylated complex from Rat Elite Kit, VECTOR; secondary antibody is biotinylated anti-Rat IgG (H+L) made in rabbit, mouse adsorbed, VECTOR
Sheep e. Sheep Elite Kit, VECTOR
TSA Tyramide Signal Amplification, PerkinElmer Life Science, Inc.

Antibody Manufacturers

The list of manufacturers refers only to those cited in this database. It is not intended to be comprehensive.
 
Lifespan Biosciences Lifespan
Abbiotec, LLC  
 
AbD Serotec www.ab-direct.com
ABGENT www.abgent.com
Abnova  
Acris  
Active Motif www.activemotif.com
Affibody www.affibody.com
Affinity BioReagents, Inc. www.bioreagents.com
Alexis Biochemicals www.alexis-e.biz
AVES Labs, Inc www.aveslab.com
Aviva Systems Biology www.avivasysbio.com
BD Biosciences www.bdbiosciences.com
Bethyl Laboratories www.bethyl.com
Biocare Medical, LLC www.biocare.net
Biocheck www.biocheckinc.com
Biogenesis, Inc. www.biogenesis.co.uk
BioGenex www.biogenex.com
BioLegend  
BIOMOL Research Laboratories, Inc. www.biomol.com
Bio-Rad http://www.bio-rad.com/
BioSource International, Inc. biosource.com
Bioss  
CALBIOCHEM www.calbiochem.com
CALTAG Laboratories www.caltag.com
Capralogics, Inc. www.capralogics.com
Cayman Chemical www.caymanchem.com
CEDARLANE Laboratories Limited www.cedarlanelabs.com
CELL Applications www.cellapplications.com
Cell Signaling Technology, Inc. www.cellsignal.com
CHEMICON International, Inc. www.chemicon.com
Cortex Biochem, Inc. www.cortex-biochem.com
Covance Research Products, Inc. www.CRPinc.com
DAKO Corporation www.dakousa.com
Diagenode  
Endogen www.endogen.com
Enzo/ Alexis  
Epitomics www.epitomics.com
Evrogen www.evrogen.com
Exalpha Biologicals, Inc. www.exalpha.com
EY Laboratories  
GeneTex, Inc. www.genetex.com
Gentest, see BD Biosciences  
GenWay Biotech, Inc. www.genwaybio.com
ICN Biomedicals, Inc., see MP Biomedicals  
ICN/Cappel Biomedicals, Inc., see MP Biomedicals  
IMGENEX  
Immunology Consultant Laboratory, Inc. www.icllab.com
ImmunoStar Incorporated www.immunostar.com
Invitrogen Life Technologies www.invitrogen.com
JaICA (Japan Institute For The Control of Aging) www.jaica.com
KAMIYA Biomedical Company www.kamiyabiomedical.com
Leica Biosystems www.leicabiosystems.com
Lifespan Biosciences  
Millipore  
Miltenyi Biotec www.miltenyibiotec.com
Molecular Biotechnology www.mobitec.com
Molecular Probes, Inc. www.probes.com
MP Biomedicals www.mpbio.com
Natural Pharmacia International tlee@natural-pharma.com
NeoMarkers, Inc. www.labvision.com
Noncommercial  
Novocastra Laboratories Ltd. www.novocastra.co.uk
Novus Biologicals, Inc. www.novus-biologicals.com
Oncogene Research Products www.apoptosis.com
Origene www.origene.com
PharMingen, see BD Biosciences  
Promega Corporation www.promega.com
ProSci Inc. www.prosci-inc.com
ProteinTech Group, Inc. www.ptglab.com
R&D Systems, Inc. www.rndsystems.com
Research Diagnostics, Inc. www.researchd.com
Roche Applied Science Roche Diagnostic Corporation www.roche-applied-science.com
Rockland www.rockland-inc.com
Santa Cruz Biotechnology, Inc. www.scbt.com
Serotec www.serotec.co.uk
Sigma www.sigma-aldrich.com
Signet Laboratories, Inc. www.signetlabs.com
Southern Biotechnology Associates, Inc. www.southernbiotech.com
 
The Binding Site www.bindingsite.com
Thermo Scientific www.thermo.com/pierce
Torry Pines Biolabs, Inc. www.chemokine.com
Transduction Laboratories, see BD Biosciences  
Upstate www.upstatebiotech.com
US Biological www.usbio.net
VECTOR Laboratories, Inc. www.vectorlabs.com
Ventana  
VMRD, Inc. www.vmrd.com
WAKO  
Zyagen  
Zymed Laboratories, Inc. www.zymed.com

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