Skip NavigationSkip to Content
Return Return to Search Results

A Direct, Efficient Method for the Preparation of siRNAs Containing Ribo-like North Bicyclo[3.1.0]hexane Pseudosugars

  1. Author:
    Terrazas, M.
    Avino, A.
    Siddiqui, M. A.
    Marquez, V. E.
    Eritja, R.

  2. Author Address

    [Siddiqui, MA; Marquez, VE] NCI, Biol Chem Lab, Ctr Canc Res, Frederick, MD 21702 USA [Terrazas, M; Avino, A; Eritja, R] Spanish Res Council CSIC, Inst Res Biomed IRB Barcelona, Networking Ctr Bioengn Biomat & Nanomed CIBER BBN, E-08028 Barcelona, Spain Spanish Res Council CSIC, Inst Adv Chem Catalonia IQAC, E-08028 Barcelona, Spain;Marquez, VE (reprint author), NCI, Biol Chem Lab, Ctr Canc Res, Frederick, MD 21702 USA;marquezv@mail.nih.gov recgma@cid.csic.es
    1. Year: 2011
    2. Date: Jun
  2. Journal: Organic Letters
    1. 13
    2. 11
    3. Pages: 2888-2891
  4. Type of Article: Article
  5. ISSN: 1523-7060
  1. Abstract:

    An efficient method for the preparation of siRNAs modified with ribo-like North bicyclo[3.1.0]hexane pseudosugars is described. The combined use of 2'-O-(2-cyanoethoxymethyl) (CEM) and 2'-O-TBDMS protection was successfully employed for RNA synthesis with the added advantage that both groups were efficiently removed in a single step. The resulting North ribo-methanocarba-modified siRNAs are compatible with the intracellular RNAi machinery and can mediate specific degradation of target mRNA.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1021/ol200909j
  3. View record in Web of Science®
  4. WOS: 000291128500024

Library Notes

  1. Fiscal Year: FY2010-2011
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel