Skip NavigationSkip to Content
Return Return to Search Results

A Reproducible and High-Throughput HPLC/MS Method To Separate Sarcosine from alpha- and beta-Alanine and To Quantify Sarcosine in Human Serum and Urine

  1. Author:
    Meyer, T. E.
    Fox, S. D.
    Issaq, H. J.
    Xu, X.
    Chu, L. W.
    Veenstra, T. D.
    Hsing, A. W.

  2. Author Address

    [Meyer, TE; Chu, LW; Hsing, AW] NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA [Fox, SD; Issaq, HJ; Xu, X; Veenstra, TD] NCI, Lab Prote & Analyt Technol, Adv Technol Program, SAIC Frederick Inc, Frederick, MD 21701 USA;Meyer, TE (reprint author), NCI, Div Canc Epidemiol & Genet, Bethesda, MD 20892 USA;Tamra.E.Meyer@us.army.mil foxsd@mail.nih.gov
    1. Year: 2011
    2. Date: Jul
  2. Journal: Analytical Chemistry
    1. 83
    2. 14
    3. Pages: 5735-5740
  4. Type of Article: Article
  5. ISSN: 0003-2700
  1. Abstract:

    While sarcosine was recently identified as a potential urine biomarker for prostate cancer, further studies have cast doubt on its utility to diagnose this condition. The inconsistent results may be due to the fact that alanine and sarcosine coelute on an HPLC reversed-phase column and the mass spectrometer cannot differentiate between the two isomers, since the same parent/product ions are generally used to measure them. In this study, we developed a high-throughput liquid chromatography-mass spectrometry (LC-MS) method that resolves sarcosine from alanine isomers, allowing its accurate quantification in human serum and urine. Assay reproducibility was determined using the coefficient of variation (CV) and intraclass correlation coefficient (ICC) in serum aliquots from 10 subjects and urine aliquots from 20 subjects across multiple analytic runs. Paired serum/urine samples from 42 subjects were used to evaluate sarcosine serum/urine correlation. Both urine and serum assays gave high sensitivity (limit of quantitation of 5 ng/mL) and reproducibility (serum assay, intra- and interassay CVs < 3% and ICCs > 99%; urine assay, intra-assay CV = 7.7% and ICC = 98.2% and interassay CV = 12.3% and ICC = 94.2%). In conclusion, this high-throughput LC-MS method is able to resolve sarcosine from alpha- and beta-alanine and is useful for quantifying sarcosine in serum and urine samples.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1021/ac201003r
  3. View record in Web of Science®
  4. WOS: 000292892000036

Library Notes

  1. Fiscal Year: FY2010-2011
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel