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Separation of High Molecular Weight Saponins of Archidendron Ellipticum By Hydrophilic Interaction Chromatography

  1. Author:
    Beutler, J. A.

  2. Author Address

    Beutler JA NCI FREDERICK CANC RES & DEV CTR LAB DRUG DISCOVERY RES & DEV DEV THERAPEUT PROGRAM FREDERICK, MD 21702 USA
    1. Year: 1997
  2. Journal: Journal of Liquid Chromatography & Related Technologies
    1. 20
    2. 15
    3. Pages: 2415-2426
  4. Type of Article: Article
  1. Abstract:

    Saponins of high molecular weight (Mr 2,000-2,100) from several species in the plant family Leguminosae were separated at both analytical and preparative scale using wide pore aminopropyl HPLC with acetonitrile-water mixtures, and gradient wide-pore butyl reversed-phase HPLC. The basis for the aminopropyl separation appears to be hydrophilic interaction chromatography, and the pore size of the media appears to play a key role in the separation. [References: 13]

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