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Two-color in situ hybridization of whole-mount mouse embryos

  1. Author:
    Biris, K. K.
    Yamaguchi, T. P.

  2. Author Address

    Frederick National Lab of Cancer Research, NCI, NIH, Frederick, MD, USA.
    1. Year: 2014
    2. Epub Date: 12/10/2013
  2. Journal: Methods in molecular biology (Clifton, N.J.)
    1. 1092
    2. Pages: 17-30
  4. Type of Article: Article
  5. ISSN: 1940-6029 (Electronic) 1064-3745 (Linking)
  1. Abstract:

    RNA in situ hybridization is a powerful technique used to identify the spatial localization of a specific RNA in a tissue section or whole tissue. In this protocol, we describe a reliable method for two-color in situ hybridization that can be used to accurately assess the expression of multiple genes with contrasting or overlapping expression patterns in whole mouse embryos.

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External Sources

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  2. DOI: 10.1007/978-1-60327-292-6_2
  3. PMID: 24318811

Library Notes

  1. Fiscal Year: FY2013-2014
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