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Genotyping of high-risk anal human papillomavirus (HPV): ion torrent-next generation sequencing vs. linear array

  1. Author:
    Nowak, Rebecca G.
    Ambulos, Nicholas P.
    Schumaker, Lisa M.
    Mathias, Trevor J.
    White, Ruth A.
    Troyer, Jennifer
    Wells, David
    Charurat, Manhattan E.
    Bentzen, Soren M.
    Cullen, Kevin J.

  2. Author Address

    Univ Maryland, Sch Med, Inst Human Virol, Baltimore, MD 21201 USA.Univ Maryland, Sch Med, Univ Maryland Marlene & Stewart Greenebaum Canc C, 22 S Green St,N9E17, Baltimore, MD 21201 USA.Columbia Univ, Dept Med Hematol & Oncol, New York, NY USA.Natl Human Genome Res Inst, Rockville, MD USA.Frederick Natl Lab Canc Res, Canc Res Technol Program, Frederick, MD USA.
    1. Year: 2017
    2. Date: Jun 13
  2. Journal: Virology Journal
  3. BIOMED CENTRAL LTD,
    1. 14
    2. 1
    3. Pages: 112
  5. Type of Article: Article
  6. Article Number: 112
  7. ISSN: 1743-422X
  1. Abstract:

    Background: Our next generation sequencing (NGS)-based human papillomavirus (HPV) genotyping assay showed a high degree of concordance with the Roche Linear Array (LA) with as little as 1.25 ng formalin-fixed paraffin-embedded-derived genomic DNA in head and neck and cervical cancer samples. This sensitive genotyping assay uses barcoded HPV PCR broad-spectrum general primers 5+/6+ (BSGP)5+/6+ applicable to population studies, but it's diagnostic performance has not been tested in cases with multiple concurrent HPV infections.

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External Sources

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  2. DOI: 10.1186/s12985-017-0771-z
  3. PMID: 28610586
  4. PMCID: PMC5470268
  5. View record in Web of Science®
  6. WOS: 000403639000001

Library Notes

  1. Fiscal Year: FY2016-2017
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