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Cdna Cloning, Expression Analysis, and Chromosomal Localization of a Gene With High Homology to Wheat Eif-(Iso)4f and Mammalian Eif-4g

  1. Author:
    Shaughnessy, J. D.
    Jenkins, N. A.
    Copeland, N. G.

  2. Author Address

    Copeland NG NCI FREDERICK CANC RES & DEV CTR MAMMALIAN GENET LAB ABL BASIC RES PROGRAM POB B FREDERICK, MD 21702 USA NCI FREDERICK CANC RES & DEV CTR MAMMALIAN GENET LAB ABL BASIC RES PROGRAM FREDERICK, MD 21702 USA
    1. Year: 1997
  2. Journal: Genomics
    1. 39
    2. 2
    3. Pages: 192-197
  4. Type of Article: Article
  1. Abstract:

    A novel mammalian gene, Eif4g2, with a high degree of homology to the p82 subunit of the wheat germ eukaryotic translation initiation factor eIF-(iso)4F and mammalian eIF-4G has been isolated. Zoo blot analysis indicates that Eif4g2 is a single-copy gene that is highly conserved among vertebrates. Northern blot analysis shows that Eif4g2 is ubiquitously expressed at high levels in all human and mouse tissues examined. The 3810-nucleotide Eif4g2 cDNA contains a 907-amino-acid open reading frame that codes for a polypeptide with a predicted molecular mass of 102 kDa. The Eif4g2 polypeptide exhibits an overall similarity to wheat p82 of 52%. A 248-amino-acid segment at the amino-terminal end of both peptides exhibits 63% similarity and contains conserved potential RNA binding domains and a phosphorylation site. The Eif4g2 polypeptide contains multiple potential N-linked glycosylation sites as well as protein kinase C and casein kinase II phosphorylation sites. Southern blot analysis of DNA from interspecific backcross mice shows that Eif4g2 is localized to distal mouse chromosome 7 in a region syntenic with human chromosome 11p15. (C) 1997 Academic Press [References: 25]

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