Skip NavigationSkip to Content
Return Return to Search Results

Prolonged evolution of the memory B cell response induced by a replicating adenovirus-influenza H5 vaccine

  1. Author:
    Matsuda, Kenta
    Huang, Jinghe
    Zhou, Tongqing
    Sheng, Zizhang
    Kang, Byong H.
    Ishida, Elise
    Griesman, Trevor
    Stuccio, Sarah
    Bolkhovitinov, Lyuba
    Wohlbold, Teddy J.
    Chromikova, Veronika
    Cagigi, Alberto
    Leung, Kwanyee
    Andrews, Sarah
    Cheung, Crystal S. F.
    Pullano, Alyssa A.
    Plyler, Jason
    Soto, Cinque
    Zhang, Baoshan
    Yang, Yongping
    Joyce, M. Gordon
    Tsybovsky,Yaroslav
    Wheatley, Adam
    Narpala, Sandeep R.
    Guo, Yicheng
    Darko, Sam
    Bailer, Robert T.
    Poole, April
    Liang, C. Jason
    Smith, Jon
    Alexander, Jeff
    Gurwith, Marc
    Migueles, Stephen A.
    Koup, Richard A.
    Golding, Hana
    Khurana, Surender
    McDermott, Adrian B.
    Shapiro, Lawrence
    Krammer, Florian
    Kwong, Peter D.
    Connors, Mark

  2. Author Address

    NIH, HIV Specif Immun Sect, Lab Immunoregulat, Bethesda, MD 20892 USA.NIAID, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA.Columbia Univ, Zukerman Inst Mind Brain Behav, Dept Biochem & Mol Biophys, New York, NY 10032 USA.Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY 10029 USA.Frederick Natl Lab Canc Res, Leidos Biomed Res Inc, Canc Res Technol Program, Electron Microscopy Lab, Frederick, MD 21701 USA.NIAID, Biostat Res Branch, NIH, Bethesda, MD 20892 USA.Emergent Biosolut Inc, Gaithersburg, MD 20879 USA.Ctr Biol Evaluat & Res, Div Viral Prod, Food & Drug Adm, Silver Spring, MD 20993 USA.
    1. Year: 2019
    2. Date: Apr 19
  2. Journal: SCIENCE IMMUNOLOGY
  3. AMER ASSOC ADVANCEMENT SCIENCE,
    1. 4
    2. 34
  5. Type of Article: Article
  6. Article Number: eaau2710
  7. ISSN: 2470-9468
  1. Abstract:

    Induction of an antibody response capable of recognizing highly diverse strains is a major obstacle to the development of vaccines for viruses such as HIV and influenza. Here, we report the dynamics of B cell expansion and evolution at the single-cell level after vaccination with a replication-competent adenovirus type 4 recombinant virus expressing influenza H5 hemagglutinin. Fluorescent H1 or H5 probes were used to quantitate and isolate peripheral blood B cells and their antigen receptors. We observed increases in H5-specific antibody somatic hypermutation and potency for several months beyond the period of active viral replication that was not detectable at the serum level. Individual broad and potent antibodies could be isolated, including one stem-specific antibody that is part of a new multidonor class. These results demonstrate prolonged evolution of the B cell response for months after vaccination and should be considered in efforts to evaluate or boost vaccine-induced immunity.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1126/sciimmunol.aau2710
  3. PMID: 31004012
  4. View record in Web of Science®
  5. WOS: 000466971900002

Library Notes

  1. Fiscal Year: FY2018-2019
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel