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Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled

  1. Author:
    Lee,Moonsup
    Hwang,Yoo-Seok
    Yoon,Jaeho
    Sun,Jian
    Harned,Adam
    Nagashima,Kunio
    Daar,Ira [ORCID]

  2. Author Address

    National Cancer Institute, Frederick, MD., Electron Microscope Laboratory, Frederick National Laboratory for Cancer Research, Frederick, MD., National Cancer Institute, Frederick, MD daari@mail.nih.gov.,
    1. Year: 2019
    2. Date: AUG
    3. Epub Date: 2019 07 03
  2. Journal: The Journal of cell biology
    1. 218
    2. 8
    3. Pages: 2659-2676
  4. Type of Article: Article
  5. ISSN: 0021-9525
  1. Abstract:

    Cilia are critical for proper embryonic development and maintaining homeostasis. Although extensively studied, there are still significant gaps regarding the proteins involved in regulating ciliogenesis. Using the Xenopus laevis embryo, we show that Dishevelled (Dvl), a key Wnt signaling scaffold that is critical to proper ciliogenesis, interacts with Drg1 (developmentally regulated GTP-binding protein 1). The loss of Drg1 or disruption of the interaction with Dvl reduces the length and number of cilia and displays defects in basal body migration and docking to the apical surface of multiciliated cells (MCCs). Moreover, Drg1 morphants display abnormal rotational polarity of basal bodies and a decrease in apical actin and RhoA activity that can be attributed to disruption of the protein complex between Dvl and Daam1, as well as between Daam1 and RhoA. These results support the concept that the Drg1-Dvl interaction regulates apical actin polymerization and stability in MCCs. Thus, Drg1 is a newly identified partner of Dvl in regulating ciliogenesis. © 2019 Lee et al.

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External Sources

  1. View Full Text
  2. DOI: 10.1083/jcb.201811147
  3. PMID: 31270137
  4. View record in Web of Science®
  5. WOS: 000478788200017
  6. PII : jcb.201811147

Library Notes

  1. Fiscal Year: FY2018-2019
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