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TRAF6-Mediated Inflammatory Cytokines Secretion in LPS-induced Colorectal Cancer Cells Is Regulated by miR-140

  1. Author:
    Zhu, Guangwei
    Lin, Chunlin
    Cheng, Zhibin
    Wang, Qin
    Hoffman, Robert M
    Singh,Shree Ram
    Huang, Yongjian
    Zheng, Wei
    Yang, Shugang
    Ye, Jianxin

  2. Author Address

    Department of Gastrointestinal Surgery 2 Section, The First Hospital Affiliated to Fujian Medical University, Fuzhou, P.R. China., Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Fujian Medical University, Fuzhou, P.R. China., AntiCancer, Inc., San Diego, CA, U.S.A., Department of Surgery, University of California, San Diego, CA, U.S.A., Basic Research Laboratory, National Cancer Institute, Frederick, MD, U.S.A. yejianxinfuyi@126.com singhshr@mail.nih.gov., Department of Gastrointestinal Surgery 2 Section, The First Hospital Affiliated to Fujian Medical University, Fuzhou, P.R. China yejianxinfuyi@126.com singhshr@mail.nih.gov.,
    1. Year: 2020
    2. Date: Jan-Feb
  2. Journal: Cancer genomics & proteomics
    1. 17
    2. 1
    3. Pages: 23-33
  4. Type of Article: Article
  5. ISSN: 1109-6535
  1. Abstract:

    Colorectal cancer (CRC) cells secrete inflammatory cytokines that affect CRC progression. The aim of the present study was to determine if micro-RNA-140(miR-140) regulates inflammatory cytokine secretion induced by lipopolysaccharide (LPS) in colorectal cancer cells by targeting tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6). Fifty fresh colon-cancer specimens and normal colorectal tissues were collected from patients with CRC and tested for the expression miR-140. Human CRC cell lines SW480 and HCT116 were treated with various concentrations and times with LPS. miR-140 and mRNA expression of potentially related genes were analyzed by qPCR. Protein expression was analyzed using western blot or ELISA. Overexpression plasmids with pcDNA3.1-TRAF6, pGL4.10-wtTRAF6 and pGL4.10-mutTRAF6 were constructed. miRNA target gene prediction and a dual luciferase assay were used to analyze miR-140-targeted TRAF6. miR-140 expression was up-regulated in CRC tissues. In CRC cells, LPS could increase miR-140 expression in a time- and concentration-dependent manner. LPS increased inflammatory cytokine mRNA expression levels in SW480 and HCT116 human colon-cancer cells. miRNA-140 suppressed TRAF6 expression via targeting the 3'UTR. TRAF6 affected miR-140-mediated inflammatory cytokine expression of SW480 and HCT116 cells under LPS treatment. miR-140 regulates inflammatory cytokine secretion of LPS-induced colorectal cancer cells by targeting TRAF6. Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

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External Sources

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  2. DOI: 10.21873/cgp.20164
  3. PMID: 31882548
  4. PMCID: PMC6937127
  5. View record in Web of Science®
  6. WOS: 000504820100002
  7. PII : 17/1/23

Library Notes

  1. Fiscal Year: FY2019-2020
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