Skip NavigationSkip to Content
Return Return to Search Results

Whole-cell imaging of plasma membrane receptors by 3D lattice light-sheet dSTORM

  1. Author:
    Wäldchen, Felix
    Schlegel, Jan [ORCID]
    Götz, Ralph
    Luciano,Michael
    Schnermann,Martin
    Doose, Sören [ORCID]
    Sauer, Markus [ORCID]

  2. Author Address

    Department of Biotechnology and Biophysics, Biocenter, University of W 252;rzburg, Am Hubland, 97074, W 252;rzburg, Germany., Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD, 21702, USA., Department of Biotechnology and Biophysics, Biocenter, University of W 252;rzburg, Am Hubland, 97074, W 252;rzburg, Germany. m.sauer@uni-wuerzburg.de.,
    1. Year: 2020
    2. Date: Feb 14
    3. Epub Date: 2020 02 14
  2. Journal: Nature communications
    1. 11
    2. 1
    3. Pages: pii:10.1038/s41467-020-14731-0
  4. Type of Article: Article
  5. Article Number: 887
  6. ISSN: 2041-1723
  1. Abstract:

    The molecular organization of receptors in the plasma membrane of cells is paramount for their functionality. We combined lattice light-sheet (LLS) microscopy with three-dimensional (3D) single-molecule localization microscopy (dSTORM) and single-particle tracking to quantify the expression and distribution, and mobility of CD56 receptors on whole fixed and living cells, finding that CD56 accumulated at cell-cell interfaces. For comparison, we investigated two other receptors, CD2 and CD45, which showed different expression levels and distributions in the plasma membrane. Overall, 3D-LLS-dSTORM enabled imaging and single-particle tracking of plasma membrane receptors with single-molecule sensitivity unperturbed by surface effects. Our results demonstrate that receptor distribution and mobility are largely unaffected by contact to the coverslip but the measured localization densities are in general lower at the basal plasma membrane due to partial limited accessibility for antibodies.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1038/s41467-020-14731-0
  3. PMID: 32060305
  4. View record in Web of Science®
  5. WOS: 000564260600004
  6. PII : 10.1038/s41467-020-14731-0

Library Notes

  1. Fiscal Year: FY2019-2020
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel