Skip NavigationSkip to Content
Return Return to Search Results

Generation of Functional Mouse Hippocampal Neurons

  1. Author:
    Tomassoni Ardori,Francesco
    Hong,Zhenyi
    Fulgenzi,Gianluca
    Tessarollo,Lino

  2. Author Address

    Neural Development Section, Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, United States.,
    1. Year: 2020
    2. Date: Aug 05
  2. Journal: Bio-protocol
    1. 10
    2. 15
    3. Pages: pii: e3702.
  4. Type of Article: Article
  5. Article Number: 3702
  6. ISSN: 2331-8325
  1. Abstract:

    Primary culture of mouse hippocampal neurons is a very useful in vitro model for studying neuronal development, axonal and dendritic morphology, synaptic functions, and many other neuronal features. Here we describe a step-by-step process of generating primary neurons from mouse embryonic hippocampi (E17.5/E18.5). Hippocampal neurons generated with this protocol can be plated in different tissue culture dishes according to different experimental aims and can produce a reliable source of pure and differentiated neurons in less than one week. This protocol covers all the steps necessary for the preparation, culture and characterization of the neuronal culture, including the illustration of dissection instruments, surgical procedure for embryos 39; isolation, culturing conditions and assessment of culture 39;s purity and differentiation. Evaluation of neuronal activity was performed by analysis of calcium imaging dynamics at six days in culture.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.21769/bioprotoc.3702
  3. PMID: 32984439
  4. PMCID: PMC7518151
  5. View record in Web of Science®
  6. WOS: 000583371200006

Library Notes

  1. Fiscal Year: FY2019-2020
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel