Skip NavigationSkip to Content
Return Return to Search Results

SARS-CoV-2 ferritin nanoparticle vaccines elicit broad SARS coronavirus immunogenicity

  1. Author:
    Joyce, M. Gordon
    Chen, Wei-Hung
    Sankhala, Rajeshwer S.
    Hajduczki, Agnes
    Thomas, Paul
    Choe, Misook
    Martinez, Elizabeth J.
    Chang, William C.
    Peterson, Caroline E.
    Morrison, Elaine B.
    Smith,Clayton
    Chen, Rita E.
    Ahmed, Aslaa
    Wieczorek, Lindsay
    Anderson, Alexander
    Case, James Brett
    Li, Yifan
    Oertel, Therese
    Rosado, Lorean
    Ganesh, Akshaya
    Whalen, Connor
    Carmen, Joshua M.
    Mendez-Rivera, Letzibeth
    Karch, Christopher P.
    Gohain, Neelakshi
    Villar, Zuzana
    McCurdy, David
    Beck, Zoltan
    Kim, Jiae
    Shrivastava, Shikha
    Jobe, Ousman
    Dussupt, Vincent
    Molnar, Sebastian
    Tran, Ursula
    Kannadka, Chandrika B.
    Soman, Sandrine
    Kuklis, Caitlin
    Zemil, Michelle
    Khanh, Htet
    Wu,Weimin
    Cole, Matthew A.
    Duso, Debra K.
    Kummer, Larry W.
    Lang, Tricia J.
    Muncil, Shania E.
    Currier, Jeffrey R.
    Krebs, Shelly J.
    Polonis, Victoria R.
    Rajan, Saravanan
    McTamney, Patrick M.
    Esser, Mark T.
    Reiley, William W.
    Rolland, Morgane
    de Val, Natalia
    Diamond, Michael S.
    Gromowski, Gregory D.
    Matyas, Gary R.
    Rao, Mangala
    Michael, Nelson L.
    Modjarrad, Kayvon

  2. Author Address

    Walter Reed Army Inst Res, Emerging Infect Dis Branch, Silver Spring, MD 20910 USA.Henry M Jackson Fdn Adv Mil Med, Bethesda, MD 20817 USA.Walter Reed Army Inst Res, US Mil HIV Res Program, Silver Spring, MD USA.NCI, NIH, Ctr Mol Microscopy, Ctr Canc Res, Frederick, MD 21701 USA.Leidos Biomed Res Inc, Frederick Natl Lab Canc Res, Canc Res Technol Program, Frederick, MD USA.Washington Univ, Dept Med, Sch Med, St Louis, MO 63110 USA.Washington Univ, Dept Pathol & Immunol, Sch Med, St Louis, MO 63110 USA.Washington Univ, Dept Mol Microbiol, Sch Med, St Louis, MO 63110 USA.Washington Univ, Andrew M & Jane M Bursky Ctr Human Immunol & Immu, Sch Med, St Louis, MO 63110 USA.Walter Reed Army Inst Res, Viral Dis Branch, Silver Spring, MD USA.Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA.Trudeau Inst Inc, Saranac Lake, NY USA.AstraZeneca, BioPharmaceut R&D, Antibody Discovery & Prot Engn ADPE, Gaithersburg, MD USA.AstraZeneca, BioPharmaceut R&D, Microbial Sci, Gaithersburg, MD USA.Walter Reed Army Inst Res, Ctr Infect Dis Res, Silver Spring, MD USA.
    1. Year: 2021
    2. Date: DEC 21
  2. Journal: CELL REPORTS
  3. CELL PRESS,
    1. 37
    2. 12
  5. Type of Article: Article
  6. Article Number: 110143
  7. ISSN: 2211-1247
  1. Abstract:

    The need for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) next-generation vaccines has been highlighted by the rise of variants of concern (VoCs) and the long-term threat of emerging coronaviruses. Here, we design and characterize four categories of engineered nanoparticle immunogens that recapitulate the structural and antigenic properties of the prefusion SARS-CoV-2 spike (S), S1, and receptor-binding domain (RBD). These immunogens induce robust S binding, ACE2 inhibition, and authentic and pseudovirus neutralizing antibodies against SARS-CoV-2. A spike-ferritin nanoparticle (SpFN) vaccine elicits neutralizing titers (ID50 > 10,000) following a single immunization, whereas RBD-ferritin nanoparticle (RFN) immunogens elicit similar responses after two immunizations and also show durable and potent neutralization against circulating VoCs. Passive transfer of immunoglobulin G (IgG) purified from SpFN- or RFN-immunized mice protects K18-hACE2 transgenic mice from a lethal SARS-CoV-2 challenge. Furthermore, S-domain nanoparticle immunization elicits ACE2-blocking activity and ID50 neutralizing antibody titers >2,000 against SARS-CoV-1, highlighting the broad response elicited by these immunogens.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1016/j.celrep.2021.110143
  3. PMID: 34919799
  4. PMCID: PMC8651551
  5. View record in Web of Science®
  6. WOS: 000734469900007

Library Notes

  1. Fiscal Year: FY2021-2022
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel