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Synthesis of [7-15N]-GTPs for RNA structure and dynamics by NMR spectroscopy

  1. Author:
    Taiwo, Kehinde M
    Olenginski, Lukasz T
    Nußbaumer, Felix
    Nam,Hyeyeon
    Hilber, Stefan
    Kreutz, Christoph
    Dayie, T Kwaku [ORCID]

  2. Author Address

    Department of Chemistry and Biochemistry, Center for Biomolecular Structure and Organization, University of Maryland, College Park, MD 20742 USA., Institute of Organic Chemistry and Center for Molecular Biosciences Innsbruck, University of Innsbruck, Innrain 80/82, 6020 Innsbruck, Austria., Present Address: Center for Cancer Research, National Cancer Institute, Frederick, MD 21702 USA.,
    1. Year: 2022
    2. Epub Date: 2022 02 26
  2. Journal: Monatshefte fur Chemie
    1. 153
    2. 3
    3. Pages: 293-299
  4. Type of Article: Article
  1. Abstract:

    Several isotope-labeling strategies have been developed for the study of RNA by nuclear magnetic resonance (NMR) spectroscopy. Here, we report a combined chemical and enzymatic synthesis of [7-15N]-guanosine-5'-triphosphates for incorporation into RNA via T7 RNA polymerase-based in vitro transcription. We showcase the utility of these labels to probe both structure and dynamics in two biologically important RNAs. The online version contains supplementary material available at 10.1007/s00706-022-02892-1. © The Author(s) 2022.

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External Sources

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  2. DOI: 10.1007/s00706-022-02892-1
  3. PMID: 35400760
  4. PMCID: PMC8948113
  5. PII : 2892

Library Notes

  1. Fiscal Year: FY2021-2022
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