Hepatic leukemia factor-expressing paraxial mesoderm cells contribute to the developing brain vasculature

Author:

Koui, Yuta

Ideue, Takako

Boylan,Michael

Anderson,Matthew

Osato, Motomi [ORCID]

Suda, Toshio

Yokomizo, Tomomasa

Mukouyama, Yoh-Suke [ORCID]
Author Address

Laboratory of Stem Cell and Neuro-Vascular Biology, Cell and Development Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA., International Research Center for Medical Sciences, Kumamoto University, Kumamoto 860-0811, Japan., Cancer and Developmental Biology Laboratory, National Cancer Institute, National Institutes of Health, Frederick, Maryland 21702, USA., Cancer Science Institute of Singapore, National University of Singapore, Singapore 117599, Singapore., Microscopic and Developmental Anatomy, Tokyo Women 39;s Medical University, Tokyo 162-8666, Japan.,

Abstract:

Recent genetic lineage tracing studies reveal heterogeneous origins of vascular endothelial cells and pericytes in the developing brain vasculature, despite classical experimental evidence for a mesodermal origin. Here we provide evidence through a genetic lineage tracing experiment that cephalic paraxial mesodermal cells give rise to endothelial cells and pericytes in the developing mouse brain. We show that Hepatic leukemia factor (Hlf) is transiently expressed by cephalic paraxial mesenchyme at embryonic day (E) 8.0-9.0 and the genetically-marked E8.0 Hlf-expressing cells mainly contribute to the developing brain vasculature. Interestingly, the genetically-marked E10.5 Hlf-expressing cells, which have been previously reported to contain embryonic hematopoietic stem cells, fail to contribute to the vascular cells. Combined, our genetic lineage tracing data demonstrate that a transient expression of Hlf marks a cephalic paraxial mesenchyme contributing to the developing brain vasculature. © 2022. Published by The Company of Biologists Ltd.

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