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Analysis of Perillic Acid in Plasma By Reversed-Phase High-Performance Liquid Chromatography With Ultraviolet Detection

  1. Author:
    Ezennia, E. I.
    Phillips, L. R.
    Wolfe, T. L.
    Tabibi, S. E.

  2. Author Address

    Ezennia EI NCI DRUG FORMULAT LAB SCI APPLICAT INT CO FREDERICK CANC RES & DEV CTR BLDG 562 ROOM 101 FREDERICK, MD 21702 USA NCI LAB PHARMACEUT CHEM DIV CANC BIOL DIAG & CTR FREDERICK CANC RES & DEV CTR FREDERICK, MD 21702 USA NIH PHARMACEUT RESOURCES BRANCH BETHESDA, MD 20892 USA
    1. Year: 1997
  2. Journal: Journal of Chromatography B
    1. 688
    2. 2
    3. Pages: 354-358
  4. Type of Article: Article
  1. Abstract:

    A simple and sensitive isocratic high-performance liquid chromatographic (HPLC) method with UV detection for the quantitation of perillic acid, a major circulating metabolite of perillyl alcohol and d-limonene, in plasma is described. Sample preparation involved protein precipitation and subsequent transfer and dilution with 10 mM NaHCO3. The mobile phase consisted of acetonitrile (36%) and 0.05 M ammonium acetate buffer pH 5.0 (64%). Separations were achieved on a C-18. column and the effluent monitored for UV absorption at the analytes' respective UVmax. Separation was excellent with no interference from endogenous plasma constituents. This method was found suitable for quantifying drug concentrations in the range of 0.25 to 200.0 mu g/ml using a 0.05-ml plasma sample, and was used to study the plasma pharmacokinetics of perillic acid in mice. [References: 7]

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