Skip NavigationSkip to Content
Return Return to Search Results

Molecular cloning and characterisation of GPR74 a novel G-protein coupled receptor closest related to the Y-receptor family

  1. Author:
    Parker, R. M. C.
    Copeland, N. G.
    Eyre, H. J.
    Liu, M.
    Gilbert, D. J.
    Crawford, J.
    Couzens, M.
    Sutherl, G. R.
    Jenkins, N. A.
    Herzog, H.

  2. Author Address

    Herzog H St Vincents Hosp, Neurobiol Program, Garvan Inst Med Res 384 Victoria St Sydney NSW 2010 Australia St Vincents Hosp, Neurobiol Program, Garvan Inst Med Res Sydney NSW 2010 Australia Womens & Childrens Hosp, Dept Cytogenet & Mol Genet, Ctr Med Genet Adelaide SA 5006 Australia NCI, Mouse Canc Genet Program, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA
    1. Year: 2000
  2. Journal: Molecular Brain Research
    1. 77
    2. 2
    3. Pages: 199-208
  4. Type of Article: Article
  1. Abstract:

    A novel gene product, GPR74, with homology to the seven transmembrane-domain receptor superfamily, has been cloned. GPR74 has been identified from the expressed sequence tags (EST) database. Subsequent PCR amplification of that sequence and screening of a human heart cDNA library led to the isolation of a 1.7-kb cDNA clone encoding a protein of 408 amino acids. GPR74 shows highest amino acid identity (33%) to the human neuropeptide Y-receptor subtype Y2. The human and mouse genes for GPR74 have been isolated and their exon-intron structures determined. In both species the gene consists of four exons spanning around 20 kb with the exon-intron borders being 100% conserved. Northern analysis of various human tissues reveals highest levels of mRNA expression in brain and heart. In situ hybridisation analysis of rat brain tissue confirms this result and identifies the hippocampus and amygdala nuclei as the brain areas with particular high expression of GPR74 mRNA. Fluorescence in situ hybridisation, PCR analysis on a radiation hybrid panel and interspecific mouse backcross mapping have localised the genes to human chromosome 4q21 and mouse chromosome 5. Expression of the human GPR74 cDNA as a GFP-fusion protein in various cell lines reveals the inability of the recombinant receptor protein to reach the cell surface. This is consistent with the lack of NPY specific binding in these cells and suggests that unknown factors are required for a full functional receptor complex. (C) 2000 Elsevier Science B.V. All rights reserved. [References: 24]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel