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Highly immunogenic DNA/LION nanocarrier vaccine potently activates lymph nodes inducing long-lasting immunity in macaques

  1. Author:
    Moussa,Maha
    Moussa,Maha
    Rosati,Margherita
    Devasundaram, Santhi
    Sengupta, Soumya
    Goldfarbmuren,Katherine
    Burns,Robert
    Bear,Jenifer
    Stellas, Dimitris
    Urban,Elizabeth
    Deleage,Claire
    Khandhar, Amit P
    Erasmus, Jesse
    Berglund, Peter
    Reed, Steven G
    Pavlakis,George
    Felber,Barbara

  2. Author Address

    Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA., Basic Science Program, Leidos Biomedical Research, Inc., Frederick, MD, USA., Advanced Biomedical Computational Science, Leidos Biomedical Research, Inc., Frederick, MD, USA., Vaccine Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA., AIDS and Cancer Virus Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA., HDT Bio Corp, Seattle, WA, USA.,
    1. Year: 2025
    2. Date: Apr 18
    3. Epub Date: 2025 03 18
  2. Journal: iScience
    1. 28
    2. 4
    3. Pages: 112232
  4. Type of Article: Article
  5. Article Number: 112232
  1. Abstract:

    A SARS-CoV-2 spike DNA vaccine formulated with a cationic nanoparticle emulsion (LION) was tested in Rhesus macaques. It induced robust, long-lasting (>2 years) cellular and humoral immunity, including increased neutralization breadth. T 160;cell responses were predominantly CD8+, in contrast to other DNA vaccines. A rapid transient cytokine/chemokine response was associated with expansion and trafficking of myeloid cells and lymphocytes. Increased proliferation and dynamic changes between blood and lymph node (LN) were found for monocyte-derived cells, dendritic cells, and B and T 160;cells, resulting in activation of LN and expansion of germinal centers (GCs), likely critical in shaping long-lasting adaptive immunity. Significant GC expansion of B, CD4-, and CD8- cells, including the Tfc3 subset, reflects a balanced immune response, including antibody (Ab) development. DNA/LION vaccination activates myeloid and lymphoid cells in blood and LN and promotes effective antigen presentation, resulting in sustained antigen-specific cellular and humoral responses, emerging as an effective DNA vaccine delivery platform.

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External Sources

  1. View Full Text
  2. DOI: 10.1016/j.isci.2025.112232
  3. PMID: 40230522
  4. PMCID: PMC11994941
  5. PII : S2589-0042(25)00493-6

Library Notes

  1. Fiscal Year: FY2024-2025
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