Skip NavigationSkip to Content
Return Return to Search Results

Multidimensional multimodal instrumental separation of complex mixtures

  1. Author:
    Issaq, H. J.
    Chan, K. C.
    Janini, G. M.
    Muschik, G. M.

  2. Author Address

    Issaq HJ NCI, SAIC Frederick, Frederick Canc Res & Dev Ctr POB B Frederick, MD 21702 USA NCI, SAIC Frederick, Frederick Canc Res & Dev Ctr Frederick, MD 21702 USA
    1. Year: 2000
  2. Journal: Journal of Liquid Chromatography & Related Technologies
    1. 23
    2. 1
    3. Pages: 145-154
  4. Type of Article: Article
  1. Abstract:

    Presented here is a simple approach to the application of HPLC/CE for the separation of complex mixtures. A two-dimensional HPLC/CE instrumental setup was assembled from commercially available equipment. Fractions of the effluent from the HPLC system are collected into microtiter plates with the aid of a microfraction collector which allows the collection of samples by time, drops, or external signal (peaks). The fractions are then dried under vacuum at room temperature in a special unit, reconstituted, and analyzed by CZE. Any size or type of HPLC or CE column can be used with no limitation on the amount of sample injected into the HPLC. Any CE detection procedure, such as LIF, MS, UV, or other, can be used. This setup is practical, simple, robust, and allows the separation of complex mixtures. Preliminary results show the utility of this system for the analysis of a mixture of two protein digests, cytochrome C and myoglobin. [References: 9]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel