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A complete system for identifying inhibitors of creatine kinase B

  1. Author:
    Towler, E. M.
    Wilson, L. K.
    Zhou, Y. C.
    Ma, T. S.
    Fisher, R. J.

  2. Author Address

    Towler EM NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick Frederick, MD 21702 USA NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick Frederick, MD 21702 USA Methodist Hosp Houston, TX 77030 USA Baylor Coll Med, Vet Adm Med Ctr Houston, TX 77030 USA
    1. Year: 2000
  2. Journal: Analytical Biochemistry
    1. 279
    2. 1
    3. Pages: 96-99
  4. Type of Article: Article
  1. Abstract:

    We have developed a complete system for discovery of lead compounds as inhibitors of creatine kinase B. In this article, we describe production and purification of the recombinant protein, conditions and features of an optimized high-throughput screening assay, and results of our implementation of the system using a diverse compound library. (C) 2000 Academic Press. [References: 24]

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