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Comparative Anchor Tagged Sequences (Cats) For Integrative Mapping of Mammalian Genomes

  1. Author:
    Lyons, L. A.
    Laughlin, T. F.
    Copeland, N. G.
    Jenkins, N. A.
    Womack, J. E.
    Obrien, S. J.

  2. Author Address

    Obrien SJ NCI FREDERICK CANC RES & DEV CTR LAB GENOM DIVERS BLDG 560 ROOM 21-105 FREDERICK, MD 21702 USA NCI FREDERICK CANC RES & DEV CTR LAB GENOM DIVERS FREDERICK, MD 21702 USA NCI FREDERICK CANC RES & DEV CTR BIOL CARCINOGENESIS & DEV PROGRAM SAIC FREDERICK FREDERICK, MD 21702 USA NCI FREDERICK CANC RES & DEV CTR MAMMALIAN GENET LAB ABL BASIC RES PROGRAM FREDERICK, MD 21702 USA TEXAS A&M UNIV DEPT VET PATHOBIOL COLLEGE STN, TX 77843 USA
    1. Year: 1997
  2. Journal: Nature Genetics
    1. 15
    2. 1
    3. Pages: 47-56
  4. Type of Article: Article
  1. Abstract:

    Precise comparisons of mammalian gene maps require common anchor loci as landmarks for conserved chromosomal segments. Using a computer script that automates DNA sequence database alignments. we designed 410 evolutionarily conserved primer pair sequences which are specific for anchor locus gene amplification from any mammalian species' DNA. Primer pairs were designed to span introns for polymorphism ascertainment. and to include sufficient exonic sequence (25-400 bp) to allow for gene identification. A total of 318 primer pairs were optimized for domestic cats, and 86% of the sequenced feline PCR products showed homology to the gene of primer origin. A screen of 20 mammals from 11 orders revealed that 35-52% of the 318 primers yielded a single PCR product without further optimization suggesting that nearly 75% can be optimized for any eutherian mammal. [References: 29]

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