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Integrase of Mason-Pfizer monkey virus

  1. Author:
    Snasel, J.
    Krejcik, Z.
    Jencova, V.
    Rosenberg, I.
    Ruml, T.
    Alexandratos, J.
    Gustchina, A.
    Pichova, I.

  2. Author Address

    Acad Sci Czech Republ, Inst Organ Chem & Biochem, CR-16610 Prague 6, Czech Republic. Acad Sci Czech Republ, Inst Mol Genet, CR-16610 Prague, Czech Republic. Acad Sci Czech Republ, Ctr Integrated Gen, CR-16610 Prague 6, Czech Republic. NCI, Macromol Crystallog Lab, Frederick, MD 21701 USA Pichova, I, Acad Sci Czech Republ, Inst Organ Chem & Biochem, Flemingovo Nam 2, CR-16610 Prague 6, Czech Republic
    1. Year: 2005
    2. Date: JAN
  2. Journal: Febs Journal
    1. 272
    2. 1
    3. Pages: 203-216
  4. Type of Article: Article
  1. Abstract:

    The gene encoding an integrase of Mason-Pfizer monkey virus (M-PMV) is located at the 3'-end of the pol open reading frame. The M-PMV integrase has not been previously isolated and characterized. We have now cloned, expressed, isolated, and characterized M-PMV integrase and compared its activities and primary structure with those of HIV-1 and other retroviral integrases. M-PMV integrase prefers untranslated 3'-region-derived long-terminal repeat sequences in both the 3'-processing and the strand transfer activity assays. While the 3'-processing reaction catalyzed by M-PMV integrase was significantly increased in the presence of Mn2+ and Co2+ and was readily detectable in the presence of Mg2+ and Ni2+ cations, the strand transfer activity was strictly dependent only on Mn2+. M-PMV integrase displays more relaxed substrate specificity than HIV-1 integrase, catalyzing the cleavage and the strand transfer of M-PMV and HIV-1 long-terminal repeat-derived substrates with similar efficiency. The structure-based sequence alignment of M-PMV, HIV-1, SIV, and ASV integrases predicted critical amino acids and motifs of M-PMV integrase for metal binding, interaction with nucleic acids, dimerization, protein structure maintenance and function, as well as for binding of human immunodeficiency virus type 1 and Rous avian sarcoma virus integrase inhibitors 5-CI-TEP, DHPTPB and Y-3

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External Sources

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  2. DOI: 10.1111/j.1432-1033.2004.04386.x
  3. View record in Web of Science®
  4. WOS: 000227358000018

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