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Differential roles of C/EBP beta regulatory domains in specifviuy MCP-1 and IL-6 transcription

  1. Author:
    Spooner, C. J.
    Guo, X. R.
    Johnson, P. F.
    Schwartz, R. C.

  2. Author Address

    Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA. NCI, Lab Prot Dynam & Signaling, Frederick, MD 21702 USA.;Schwartz, RC, Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA.;schwart9@msu.edu
    1. Year: 2007
    2. Date: Feb
  2. Journal: Molecular Immunology
    1. 44
    2. 6
    3. Pages: 1384-1392
  4. Type of Article: Article
  5. ISSN: 0161-5890
  1. Abstract:

    C/EBP beta is a member of the CCAAT/enhancer binding protein family of transcription factors and has been shown to be a critical transcriptional regulator of various proinflammatory genes, including IL-6 and MCP-1. To examine the roles of the C/EBP beta transactivation and regulatory domains in LPS-induced MCP-1 and IL-6 expression, we expressed various N-terminal truncations and deletions of C/EBP beta in P388 murine B lymphoblasts, which lack endogenous C/EBP beta expression and are normally unresponsive to LPS for expression of IL-6 and MCP-1. Unexpectedly, a region between amino acids 105 and 212 of C/EBP beta that includes regulatory domains I and 2 facilitates C/EBP beta activation of IL-6 expression, while having an inhibitory effect on MCP-1 expression. Thus, this region can mediate promoter-specific effects on cytokine and chemokine gene transcription. LIP, the naturally occurring truncated form of C/EBP beta, largely retains these regulatory domains and stimulates IL-6 but not MCP-1 transcription. (c) 2006 Elsevier Ltd. All rights reserved.

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External Sources

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  2. DOI: 10.1016/j.molimm.2006.05.004
  3. View record in Web of Science®
  4. WOS: 000242949700035

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