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Preparation and preliminary evaluation of a biotin-targeted, lectin-targeted dendrimer-based probe for dual-modality magnetic resonance and fluorescence Imaging

  1. Author:
    Xu, H.
    Regino, C. A. S.
    Koyama, Y.
    Hama, Y.
    Gunn, A. J.
    Bernardo, M.
    Kobayashi, H.
    Choyke, P. L.
    Brechbiel, M. W.

  2. Author Address

    NIH, NCI, Radiat Oncol Branch, Radioimmune & Inorgan Chem Sect, Bethesda, MD 20892 USA. Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick Inc, Res Technol Program, Frederick, MD 21702 USA.;Brechbiel, MW, NIH, NCI, Radiat Oncol Branch, Radioimmune & Inorgan Chem Sect, Bldg 10,Room 1B40,10Ctr Dr, Bethesda, MD 20892 USA.;martinwb@mail.nih.gov
    1. Year: 2007
    2. Date: Sep-Oct
  2. Journal: Bioconjugate Chemistry
    1. 18
    2. 5
    3. Pages: 1474-1482
  4. Type of Article: Article
  5. ISSN: 1043-1802
  1. Abstract:

    A novel approach for the preparation of a biotinylated dendrimer-based MRI agent 5 is described, in which a unique disulfide bond in the core of the Gd(III)-1B4M-DTPA chelated G2 PAMAM dendrimer was reduced and then attached to a maleimide-functionalized biotin. The new MRI agent 5 features a well-defined dendron structure and a unique biotin functionality. Immobilization of up to four copies of biotinylated dendrimer 5 to fluorescently labeled avidin yields a supramolecular avidin-biotin-dendrimer-Gd(III) complex. Validation of the complex in mice bearing ovarian cancer tumors demonstrates that the avidin-biotin-dendrimer targeting system efficiently targets and delivers sufficient amounts of chelated Gd(III) and fluorophores (e.g., Rhodamine green) to ovarian tumors to produce visible changes in the tumors by both MRI and optical imaging, respectively. Thus, the avidin-biotin-dendrimer complex may be used as a tumor-targeted probe for dual-modality magnetic resonance and fluorescence imaging.

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External Sources

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  2. DOI: 10.1021/bc0701085
  3. View record in Web of Science®
  4. WOS: 000249656100016

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