Skip NavigationSkip to Content
Return Return to Search Results

Non-equilibrium and differential function between intraepithelial and lamina propria virus-specific TCR alpha beta(+) CD8 alpha beta(+) T cells in the small intestinal mucosa

  1. Author:
    Isakov, D.
    Dzutsev, A.
    Belyakov, I. M.
    Berzofsky, J. A.

  2. Author Address

    Isakov, D.; Dzutsev, A.; Belyakov, I. M.; Berzofsky, J. A.] NCI, Mol Immunogenet & Vaccine Res Sect, Vaccine Branch, Ctr Canc Res,NIH, Bethesda, MD 20892 USA. [Belyakov, I. M.] Midwest Res Inst, Frederick, MD USA.
    1. Year: 2009
  2. Journal: Mucosal Immunology
    1. 2
    2. 5
    3. Pages: 450-461
  4. Type of Article: Article
  1. Abstract:

    The gastrointestinal mucosa regularly encounters commensal and pathogenic microbiota. Gut mucosal lymphocytes consist of two phenotypically different populations residing in the intestinal intraepithelial (IEL) compartment and lamina propria (LP). Little is known about compositional and functional differences of antigen-specific T cells from these mucosal compartments after mucosal infection, or the degree of trafficking between them. We here studied the B8R(20-27)-specific CD8 T-cell response in LP and IEL compartments after intrarectal immunization with modified vaccinia virus Ankara (MVA). CD8(+) T cells in the IEL compartment had much lower avidity than in the LP or spleen during acute and memory phases. Surprisingly, the TCR V beta-chain distribution of antigen-specific T cells and the length of the CDR3 region of the dominant V beta genes showed substantial dissimilarities between IEL and LP antigen-specific CD8 alpha beta T cells in individual mice, increasing with time. We show functional and compositional differences between these mucosal compartments during the effector and memory phases of the immune response, indicating limited crosstalk and microenvironmental differences between the IEL, LP, and spleen. The restricted migration of cells from each of these mucosal compartments could partly account for a founder effect we observed in the IEL TCR alpha beta CD8 alpha beta epitope-specific repertoire that might impact protective efficacy.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1038/mi.2009.95
  3. PMID: 19571797

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel