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Expression of Interleukin-15 and Its Receptor on the Surface of Stimulated Human Umbilical Vein Endothelial Cells

  1. Author:
    Liu, X. P.
    Zuo, Y. M.
    Zhang, W. N.
    Yang, D. G.
    Xiong, C. Y.
    Zhang, X. Z.

  2. Author Address

    Zhang, Xiaozhou] Huazhong Univ Sci & Technol, Xianggan Med Coll Hosp, Xiaogan 432000, Peoples R China. [Liu, Xiuping] Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China. [Liu, Xiuping] Hubei Vocat & Tech Coll, Xiaogan 432000, Peoples R China. [Zuo, Yumei] Third Mil Med Univ, Dept Cardiol, Southwest Hosp, Chongqing 400038, Peoples R China. [Zhang, Weina] Huazhong Univ Sci & Technol, Minist Educ,Minist Hlth, Inst Organ Transplantat,Tongji Med Coll, Tongji Hosp,Key Lab Organ Transplantat, Wuhan 430030, Peoples R China. [Yang, Deguang] NE Agr Univ, Coll Agr, Harbin 150030, Peoples R China. [Xiong, Changyun] NCI, Mol Targets Dev Program, NIH, Frederick, MD 21702 USA.
    1. Year: 2009
  2. Journal: Journal of Huazhong University of Science and Technology-medical Sciences
    1. 29
    2. 5
    3. Pages: 527-534
  4. Type of Article: Article
  1. Abstract:

    Human interleukin-15 (hIL-15) is an important cytokine to activate endothelial cells and can be regulated by many other cytokines. The aim of this study is to examine the ability of interferon-gamma (IFN-gamma), and tumor necrosis factor-alpha (TNF-alpha) to induce the production of human interleukin-15 (hIL-15) and IL-15 receptor (IL-15R alpha) by human umbilical vein endothelial cells (HUVECs). The data are summarized as follows: 1. Northern blot revealed that IL-15 mRNA was up-regulated by IFN-gamma and TNF-alpha. 2. Intracellular IL-15 protein was visualized by fluorescence microscopy, whereas the expression of IL-15 on the surface of HUVECs was detected by fluorescence activated cell sorting (FACS), and no detectable IL-15 in the medium was verified by ELISA. 3. IL-15R alpha was detected on the surface of HUVECs by FACS after IFN-gamma and TNF-alpha stimulation, whereas Western blotting revealed that the elevated expression on surface IL-15R alpha was not due to the increased protein expression. The conclusion demonstrated from our results is that IFN-gamma and TNF-alpha play an important role in regulating the expression of IL-15 and IL-15R alpha on the surface of HUVECs.

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  2. DOI: 10.1007/s11596-009-0501-x
  3. PMID: 19821081

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