Skip NavigationSkip to Content
Return Return to Search Results

Combined Blood/Tissue Analysis for Cancer Biomarker Discovery: Application to Renal Cell Carcinoma

  1. Author:
    Johann, D. J.
    Wei, B. R.
    Prieto, D. A.
    Chan, K. C.
    Ye, X. Y.
    Valera, V. A.
    Simpson, R. M.
    Rudnick, P. A.
    Xiao, Z.
    Issaq, H. J.
    Linehan, W. M.
    Stein, S. E.
    Veenstra, T. D.
    Blonder, J.

  2. Author Address

    [Prieto, DaRue A.; Chan, King C.; Ye, Xiaying; Xiao, Zhen; Issaq, Haleem J.; Veenstra, Timothy D.; Blonder, Josip] NCI, Lab Prote & Analyt Technol, Adv Technol Program, SAIC Frederick Inc, Frederick, MD 21702 USA. [Johann, Donald J., Jr.] NCI, Med Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA. [Wei, Bih-Rong; Simpson, R. Mark] NCI, Lab Canc Biol & Genet, Ctr Canc Res, Bethesda, MD 20892 USA. [Valera, Vladimir A.; Linehan, W. Marston] NCI, Urol Oncol Branch, Ctr Canc Res, Bethesda, MD 20892 USA. [Rudnick, Paul A.; Stein, Stephen E.] Natl Inst Stand & Technol, NIST Mass Spectrometry Data Ctr, Gaithersburg, MD 20899 USA.;Blonder, J, NCI, Lab Prote & Analyt Technol, Adv Technol Program, SAIC Frederick Inc, POB B, Frederick, MD 21702 USA.;blonderj@mail.nih.gov
    1. Year: 2010
    2. Date: Mar
  2. Journal: Analytical Chemistry
    1. 82
    2. 5
    3. Pages: 1584-1588
  4. Type of Article: Article
  5. ISSN: 0003-2700
  1. Abstract:

    A method that relies on subtractive tissue-directed shotgun proteomics to identify tumor proteins in the blood of a patient newly diagnosed with cancer is described. To avoid analytical and statistical biases caused by physiologic variability of protein expression in the human population, this method was applied on clinical specimens obtained from a single patient diagnosed with nonmetastatic renal cell carcinoma (RCC). The proteomes extracted from tumor, normal adjacent tissue and preoperative plasma were analyzed using 2D-liquid chromatography-mass spectrometry (LC-MS). The lists of identified proteins were filtered to discover proteins that (i) were found in the tumor but not normal tissue, (ii) were identified in matching plasma, and (iii) whose spectral count was higher in tumor tissue than plasma. These filtering criteria resulted in identification of eight tumor proteins in the blood. Subsequent Western-blot analysis confirmed the presence of cadherin-5, cadherin-11, DEAD-box protein-23, and pyruvate kinase in the blood of the patient in the study as well as in the blood of four other patients diagnosed with RCC. These results demonstrate the utility of a combined blood/tissue analysis strategy that permits the detection of tumor proteins in the blood of a patient diagnosed with RCC.

    See More

  1. Keywords:

External Sources

  1. View Full Text
  2. DOI: 10.1021/ac902204k
  3. View record in Web of Science®
  4. WOS: 000274841300005

Library Notes

  1. Fiscal Year: FY2009-2010
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel