Skip NavigationSkip to Content
Return Return to Search Results

Chemokine Production and Adhesion Molecule Expression By Neural Cells Exposed to Il-1, Tnf-Alpha and Interferon-Gamma

  1. Author:
    Chuluyan, H. E.
    Lang, B. J.
    Yoshimura, T.
    Kenney, J. S.
    Issekutz, A. C.

  2. Author Address

    Issekutz AC DALHOUSIE UNIV IWK GRACE HLTH CTR DEPT PEDIAT MICROBIOL & IMMUNOL 5850 UNIV AVE HALIFAX NS B3J 3G9 CANADA DALHOUSIE UNIV IWK GRACE HLTH CTR DEPT PEDIAT MICROBIOL & IMMUNOL HALIFAX NS B3J 3G9 CANADA NCI FREDERICK, MD 21701 USA ANTIBODY SOLUT PALO ALTO, CA USA
    1. Year: 1998
  2. Journal: Life Sciences
    1. 63
    2. 21
    3. Pages: 1939-1952
  4. Type of Article: Article
  1. Abstract:

    We investigated the effect of TNF alpha, IL-1 alpha and IFN gamma on two neuroblastoma (NB) cell lines (SK-N-SH and SK-N-MC). These lines responded differentially to IL-1 alpha, TNF alpha and IFN gamma for MCP-1 and IL-8 production and expression of the ICAM-1 and VCAM-1 adhesion molecules. None of the cytokines induced MCP-1 or IL-8 on SK-N-MC cells. Both chemokines were produced in response to IL-1 alpha by SK-N-SH cells, while TNF alpha induced mainly MCP-1 production. Addition of IFN gamma decreased IL-8, but not MCP-1 production. These responses correlated with monocyte and neutrophil chemotactic activity in NE culture supernatants. This activity was neutralized by antibodies to IL-8 and MCP-1. The expression of ICAM-1 on SK-N-MC was up-regulated by TNF alpha or IFN gamma, while IL-1 alpha also upregulated ICAM-1 on SK-N-SH cells. VCAM-1 expression on SK-N-SH was induced by IL-1 alpha and TNF alpha and IFN gamma synergized with TNF alpha in this respect on both NE cell lines. These results suggest that mechanisms for chemokine production and VCAM-1 and ICAM-1 upregulation by inflammatory cytokines differ and IFN gamma, in conjunction with TNF alpha, stimulate neural cell responses (high MCP-1 and VCAM-1 and decreased IL-8) favouring mononuclear cell recruitment. [References: 46]

    See More

  1. Keywords:

External Sources

  1. No sources found.

Library Notes

  1. No notes added.
NCI at Frederick

You are leaving a government website.

This external link provides additional information that is consistent with the intended purpose of this site. The government cannot attest to the accuracy of a non-federal site.

Linking to a non-federal site does not constitute an endorsement by this institution or any of its employees of the sponsors or the information and products presented on the site. You will be subject to the destination site's privacy policy when you follow the link.

ContinueCancel